2AQU

Structure of HIV-1 protease bound to atazanavir

2AQU の概要

• エントリーDOI: 10.2210/pdb2aqu/pdb
• 分子名称: HIV-1 Protease, (3S,8S,9S,12S)-3,12-BIS(1,1-DIMETHYLETHYL)-8-HYDROXY-4,11-DIOXO-9-(PHENYLMETHYL)-6-[[4-(2-PYRIDINYL)PHENYL]METHYL]-2,5, 6,10,13-PENTAAZATETRADECANEDIOIC ACID DIMETHYL ESTER (3 entities in total)
• 機能のキーワード: aids, aspartyl protease, hydrolase
• 由来する生物種: Human immunodeficiency virus 1
• 細胞内の位置: Matrix protein p17: Virion (Potential). Capsid protein p24: Virion (Potential). Nucleocapsid protein p7: Virion (Potential). Reverse transcriptase/ribonuclease H: Virion (Potential). Integrase: Virion (Potential): P03366
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22312.37

構造登録者

Clemente, J.C.,Coman, R.M.,Thiaville, M.M.,Janka, L.K.,Jeung, J.A.,Nukoolkarn, S.,Govindasamy, L.,Agbandje-McKenna, M.,McKenna, R.,Leelamanit, W.,Goodenow, M.M.,Dunn, B.M. (登録日: 2005-08-18, 公開日: 2006-08-29, 最終更新日: 2023-08-23)

主引用文献

Clemente, J.C.,Coman, R.M.,Thiaville, M.M.,Janka, L.K.,Jeung, J.A.,Nukoolkarn, S.,Govindasamy, L.,Agbandje-McKenna, M.,McKenna, R.,Leelamanit, W.,Goodenow, M.M.,Dunn, B.M.
Analysis of HIV-1 CRF_01 A/E protease inhibitor resistance: structural determinants for maintaining sensitivity and developing resistance to atazanavir.
Biochemistry, 45:5468-5477, 2006

PubMed Abstract: A series of HIV-1 protease mutants has been designed in an effort to analyze the contribution to drug resistance provided by natural polymorphisms as well as therapy-selective (active and non-active site) mutations in the HIV-1 CRF_01 A/E (AE) protease when compared to that of the subtype B (B) protease. Kinetic analysis of these variants using chromogenic substrates showed differences in substrate specificity between pretherapy B and AE proteases. Inhibition analysis with ritonavir, indinavir, nelfinavir, amprenavir, saquinavir, lopinavir, and atazanavir revealed that the natural polymorphisms found in A/E can influence inhibitor resistance. It was also apparent that a high level of resistance in the A/E protease, as with B protease, is due to it aquiring a combination of active site and non-active site mutations. Structural analysis of atazanavir bound to a pretherapy B protease showed that the ability of atazanavir to maintain its binding affinity for variants containing some resistance mutations is due to its unique interactions with flap residues. This structure also explains why the I50L and I84V mutations are important in decreasing the binding affinity of atazanavir.

PubMed: 16634628
DOI: 10.1021/bi051886s

実験手法

X-RAY DIFFRACTION (2 Å)