2AQE

Structural and functional analysis of ada2 alpha swirm domain

2AQE の概要

• エントリーDOI: 10.2210/pdb2aqe/pdb
• 関連するPDBエントリー: 2AQF
• NMR情報: BMRB: 6781
• 分子名称: Transcriptional adaptor 2, Ada2 alpha (1 entity in total)
• 機能のキーワード: helix-turn-helix, transcription
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10042.67

構造登録者

Qian, C.,Zhang, Q.,Zeng, L.,Zhou, M.-M. (登録日: 2005-08-17, 公開日: 2005-12-13, 最終更新日: 2024-05-22)

主引用文献

Qian, C.,Zhang, Q.,Li, S.D.,Zeng, L.,Walsh, M.J.,Zhou, M.-M.
Structure and chromosomal DNA binding of the SWIRM domain
Nat.Struct.Mol.Biol., 12:1078-1085, 2005

PubMed Abstract: The evolutionarily conserved Swi3p, Rsc8p and Moira (SWIRM) domain is found in many chromosomal proteins involved in chromatin modifications or remodeling. Here we report the three-dimensional solution structure of the SWIRM domain from the human transcriptional adaptor ADA2alpha. The structure reveals a five-helix bundle consisting of two helix-turn-helix motifs connected by a central long helix, reminiscent of the histone fold. Using structural and biochemical analyses, we showed that the SWIRM domains of human ADA2alpha and SMARC2 bind to double-stranded and nucleosomal DNA, and we identified amino acid residues required for this function. We demonstrated that the ADA2alpha SWIRM domain is colocalized with lysine-acetylated histone H3 in the cell nucleus and that it potentiates the ACF remodeling activity by enhancing accessibility of nucleosomal linker DNA bound to histone H1. These data suggest a functional role of the SWIRM domain in chromatin remodeling.

PubMed: 16299514
DOI: 10.1038/nsmb1022

実験手法

SOLUTION NMR