2ANT

THE 2.6 A STRUCTURE OF ANTITHROMBIN INDICATES A CONFORMATIONAL CHANGE AT THE HEPARIN BINDING SITE

2ANT の概要

• エントリーDOI: 10.2210/pdb2ant/pdb
• 分子名称: ANTITHROMBIN, 2-acetamido-2-deoxy-beta-D-allopyranose (3 entities in total)
• 機能のキーワード: serpin, heparin, inhibitor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted, extracellular space: P01008
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 98644.45

構造登録者

Skinner, R.,Abrahams, J.-P.,Whisstock, J.C.,Lesk, A.M.,Carrell, R.W.,Wardell, M.R. (登録日: 1997-01-28, 公開日: 1997-06-16, 最終更新日: 2024-12-25)

主引用文献

Skinner, R.,Abrahams, J.P.,Whisstock, J.C.,Lesk, A.M.,Carrell, R.W.,Wardell, M.R.
The 2.6 A structure of antithrombin indicates a conformational change at the heparin binding site.
J.Mol.Biol., 266:601-609, 1997

PubMed Abstract: The crystal structure of a dimeric form of intact antithrombin has been solved to 2.6 A, representing the highest-resolution structure of an active, inhibitory serpin to date. The crystals were grown under microgravity conditions on Space Shuttle mission STS-67. The overall confidence in the structure, determined earlier from lower resolution data, is increased and new insights into the structure-function relationship are gained. Clear and continuous electron density is present for the reactive centre loop region P12 to P14 inserting into the top of the A-beta-sheet. Areas of the extended amino terminus, unique to antithrombin and important in the binding of the glycosaminoglycan heparin, can now be traced further than in the earlier structures. As in the earlier studies, the crystals contain one active and one latent molecule per asymmetric unit. Better definition of the electron density surrounding the D-helix and of the residues implicated in the binding of the heparin pentasaccharide (Arg47, Lys114, Lys125, Arg129) provides an insight into the change of affinity of binding that accompanies the change in conformation. In particular, the observed hydrogen bonding of these residues to the body of the molecule in the latent form explains the mechanism for the release of newly formed antithrombin-protease complexes into the circulation for catabolic removal.

PubMed: 9067613
DOI: 10.1006/jmbi.1996.0798

実験手法

X-RAY DIFFRACTION (2.6 Å)