2ANS

ADIPOCYTE LIPID BINDING PROTEIN COMPLEXED WITH 1-ANILINO-8-NAPHTHALENE SULFONATE

2ANS の概要

• エントリーDOI: 10.2210/pdb2ans/pdb
• 分子名称: ADIPOCYTE LIPID-BINDING PROTEIN, CHLORIDE ION, 8-ANILINO-1-NAPHTHALENE SULFONATE, ... (4 entities in total)
• 機能のキーワード: lipid-binding protein, fluorescent probe, lipid binding protein
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29713.52

構造登録者

Ory, J.J.,Banaszak, L.J. (登録日: 1999-01-19, 公開日: 1999-07-22, 最終更新日: 2024-05-22)

主引用文献

Ory, J.J.,Banaszak, L.J.
Studies of the ligand binding reaction of adipocyte lipid binding protein using the fluorescent probe 1, 8-anilinonaphthalene-8-sulfonate.
Biophys.J., 77:1107-1116, 1999

PubMed Abstract: The fluorescent probe anilinonaphthalene-8-sulfonate binds to adipocyte lipid binding protein at a site that competes with normal physiological ligands, such as fatty acids. Binding to the protein is accompanied by a relatively large increase in fluorescent intensity. To correlate the major change in optical properties and to determine the mechanism of competitive inhibition with fatty acids, the crystal structure of the protein with the bound fluorophore has been determined. In addition, the thermodynamic contributions to the binding reaction have been studied by titration calorimetry. Because the binding site is in a relatively internal position, kinetic studies have also been carried out to determine k(on). The results indicate that binding is not accompanied by any major conformational change. However, the negatively charged sulfonate moiety is not positioned the same as the carboxylate of fatty acid ligands as determined in previous studies. Nonetheless, the binding reaction is still driven by enthalpic effects. As judged by the crystallographic structure, a significant amount of the surface of the fluorophore is no longer exposed to water in the bound state.

PubMed: 10423455

実験手法

X-RAY DIFFRACTION (2.5 Å)