2ALA

Crystal structure of the Semliki Forest Virus envelope protein E1 in its monomeric conformation.

2ALA の概要

• エントリーDOI: 10.2210/pdb2ala/pdb
• 関連するPDBエントリー: 1I9W 1RER
• 分子名称: Structural polyprotein (P130) (2 entities in total)
• 機能のキーワード: envelope glycoprotein, membrane fusion, viral protein
• 由来する生物種: Semliki forest virus
• 細胞内の位置: Capsid protein: Virion (By similarity). p62: Virion membrane; Single-pass type I membrane protein (By similarity). E2 envelope glycoprotein: Virion membrane; Single-pass type I membrane protein (By similarity). E1 envelope glycoprotein: Virion membrane; Single-pass type I membrane protein (By similarity). 6K protein: Host cell membrane; Multi-pass membrane protein (By similarity): P03315
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42690.13

構造登録者

Roussel, A.,Lescar, J.,Vaney, M.C.,Wengler, G.,Wengler, G.,Rey, F.A. (登録日: 2005-08-05, 公開日: 2006-01-17, 最終更新日: 2024-11-06)

主引用文献

Roussel, A.,Lescar, J.,Vaney, M.C.,Wengler, G.,Wengler, G.,Rey, F.A.
Structure and interactions at the viral surface of the envelope protein E1 of Semliki Forest virus.
Structure, 14:75-86, 2006

PubMed Abstract: Semliki Forest virus (SFV) is enveloped by a lipid bilayer enclosed within a glycoprotein cage made by glycoproteins E1 and E2. E1 is responsible for inducing membrane fusion, triggered by exposure to the acidic environment of the endosomes. Acidic pH induces E1/E2 dissociation, allowing E1 to interact with the target membrane, and, at the same time, to rearrange into E1 homotrimers that drive the membrane fusion reaction. We previously reported a preliminary Calpha trace of the monomeric E1 glycoprotein ectodomain and its organization on the virus particle. We also reported the 3.3 A structure of the trimeric, fusogenic conformation of E1. Here, we report the crystal structure of monomeric E1 refined to 3 A resolution and describe the amino acids involved in contacts in the virion. These results identify the major determinants for the E1/E2 icosahedral shell formation and open the way to rational mutagenesis approaches to shed light on SFV assembly.

PubMed: 16407067
DOI: 10.1016/j.str.2005.09.014

実験手法

X-RAY DIFFRACTION (3 Å)