2AIB

beta-cinnamomin in complex with ergosterol

2AIB の概要

• エントリーDOI: 10.2210/pdb2aib/pdb
• 関連するPDBエントリー: 2A8F
• 分子名称: Beta-elicitin cinnamomin, ERGOSTEROL, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (5 entities in total)
• 機能のキーワード: elicitin, sterol carrier protein, phytophthora, phytopathogen, toxin
• 由来する生物種: Phytophthora cinnamomi
• 細胞内の位置: Secreted: P15569
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 21772.24

構造登録者

Rodrigues, M.L.,Archer, M.,Martel, P.,Miranda, S.,Thomaz, M.,Enguita, F.J.,Baptista, R.P.,Melo, E.P.,Sousa, N.,Cravador, A.,Carrondo, M.A. (登録日: 2005-07-29, 公開日: 2006-01-17, 最終更新日: 2024-10-30)

主引用文献

Rodrigues, M.L.,Archer, M.,Martel, P.,Miranda, S.,Thomaz, M.,Enguita, F.J.,Baptista, R.P.,Melo, E.P.,Sousa, N.,Cravador, A.,Carrondo, M.A.
Crystal structures of the free and sterol-bound forms of beta-cinnamomin
Biochim.Biophys.Acta, 1764:110-121, 2006

PubMed Abstract: The crystal structure of the elicitin beta-cinnamomin (beta-CIN) was determined in complex with ergosterol at 1.1 A resolution. beta-CIN/ergosterol complex crystallized in the monoclinic space group P2(1), with unit cell parameters of a = 31.0, b = 62.8, c = 50.0 A and beta = 93.4 degrees and two molecules in the asymmetric unit. Ligand extraction with chloroform followed by crystallographic analysis yielded a 1.35 A structure of beta-CIN (P4(3)2(1)2 space group) where the characteristic elicitin fold was kept. After incubation with cholesterol, a new complex structure was obtained, showing that the protein retains, after the extraction procedure, its ability to complex sterols. The necrotic effect of beta-CIN on tobacco was also shown to remain unchanged. Theoretical docking studies of the triterpene lupeol to beta-CIN provided an explanation for the apparent inability of beta-CIN to bind this ligand, as observed experimentally.

PubMed: 16249127
DOI: 10.1016/j.bbapap.2005.09.008

実験手法

X-RAY DIFFRACTION (1.1 Å)