2AI9

S.aureus Polypeptide Deformylase

2AI9 の概要

• エントリーDOI: 10.2210/pdb2ai9/pdb
• 関連するPDBエントリー: 2AI7 2AI8 2AIA 2AIE
• 分子名称: Peptide deformylase, NICKEL (II) ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: hudrolase, hydrolase
• 由来する生物種: Staphylococcus aureus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 41390.60

構造登録者

Smith, K.J.,Petit, C.M.,Aubart, K.,Smyth, M.,McManus, E.,Jones, J.,Fosberry, A.,Lewis, C.,Lonetto, M.,Christensen, S.B. (登録日: 2005-07-29, 公開日: 2005-09-06, 最終更新日: 2024-02-14)

主引用文献

Smith, K.J.,Petit, C.M.,Aubart, K.,Smyth, M.,McManus, E.,Jones, J.,Fosberry, A.,Lewis, C.,Lonetto, M.,Christensen, S.B.
Structural Variation and inhibitor binding in polypeptide deformylase from four different bacterial species.
Protein Sci., 12:349-360, 2003

PubMed Abstract: Polypeptide deformylase (PDF) catalyzes the deformylation of polypeptide chains in bacteria. It is essential for bacterial cell viability and is a potential antibacterial drug target. Here, we report the crystal structures of polypeptide deformylase from four different species of bacteria: Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, and Escherichia coli. Comparison of these four structures reveals significant overall differences between the two Gram-negative species (E. coli and H. influenzae) and the two Gram-positive species (S. pneumoniae and S. aureus). Despite these differences and low overall sequence identity, the S1' pocket of PDF is well conserved among the four enzymes studied. We also describe the binding of nonpeptidic inhibitor molecules SB-485345, SB-543668, and SB-505684 to both S. pneumoniae and E. coli PDF. Comparison of these structures shows similar binding interactions with both Gram-negative and Gram-positive species. Understanding the similarities and subtle differences in active site structure between species will help to design broad-spectrum polypeptide deformylase inhibitor molecules.

PubMed: 12538898
DOI: 10.1110/ps.0229303

実験手法

X-RAY DIFFRACTION (2.5 Å)