2AHV

Crystal Structure of Acyl-CoA transferase from E. coli O157:H7 (YdiF)-thioester complex with CoA- 1

2AHV の概要

• エントリーDOI: 10.2210/pdb2ahv/pdb
• 関連するPDBエントリー: 2AHU 2AHW
• 分子名称: putative enzyme YdiF, COENZYME A (3 entities in total)
• 機能のキーワード: ydif, coa transferase, glutamyl thioester, structural genomics, montreal-kingston bacterial structural genomics initiative, bsgi, transferase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 233337.79

構造登録者

Rangarajan, E.S.,Li, Y.,Ajamian, E.,Iannuzzi, P.,Kernaghan, S.D.,Fraser, M.E.,Cygler, M.,Matte, A.,Montreal-Kingston Bacterial Structural Genomics Initiative (BSGI) (登録日: 2005-07-28, 公開日: 2005-11-01, 最終更新日: 2024-10-09)

主引用文献

Rangarajan, E.S.,Li, Y.,Ajamian, E.,Iannuzzi, P.,Kernaghan, S.D.,Fraser, M.E.,Cygler, M.,Matte, A.
Crystallographic trapping of the glutamyl-CoA thioester intermediate of family I CoA transferases.
J.Biol.Chem., 280:42919-42928, 2005

PubMed Abstract: Coenzyme A transferases are involved in a broad range of biochemical processes in both prokaryotes and eukaryotes, and exhibit a diverse range of substrate specificities. The YdiF protein from Escherichia coli O157:H7 is an acyl-CoA transferase of unknown physiological function, and belongs to a large sequence family of CoA transferases, present in bacteria to humans, which utilize oxoacids as acceptors. In vitro measurements showed that YdiF displays enzymatic activity with short-chain acyl-CoAs. The crystal structures of YdiF and its complex with CoA, the first co-crystal structure for any Family I CoA transferase, have been determined and refined at 1.9 and 2.0 A resolution, respectively. YdiF is organized into tetramers, with each monomer having an open alpha/beta structure characteristic of Family I CoA transferases. Co-crystallization of YdiF with a variety of CoA thioesters in the absence of acceptor carboxylic acid resulted in trapping a covalent gamma-glutamyl-CoA thioester intermediate. The CoA binds within a well defined pocket at the N- and C-terminal domain interface, but makes contact only with the C-terminal domain. The structure of the YdiF complex provides a basis for understanding the different catalytic steps in the reaction of Family I CoA transferases.

PubMed: 16253988
DOI: 10.1074/jbc.M510522200

実験手法

X-RAY DIFFRACTION (2 Å)