2AHO

Structure of the archaeal initiation factor eIF2 alpha-gamma heterodimer from Sulfolobus solfataricus complexed with GDPNP

2AHO の概要

• エントリーDOI: 10.2210/pdb2aho/pdb
• 分子名称: Translation initiation factor 2 gamma subunit, Translation initiation factor 2 alpha subunit, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: initiation of translation, translation
• 由来する生物種: Sulfolobus solfataricus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 76762.30

構造登録者

Yatime, L.,Mechulam, Y.,Blanquet, S.,Schmitt, E. (登録日: 2005-07-28, 公開日: 2006-01-31, 最終更新日: 2023-10-25)

主引用文献

Yatime, L.,Mechulam, Y.,Blanquet, S.,Schmitt, E.
Structural Switch of the gamma Subunit in an Archaeal aIF2alphagamma Heterodimer
Structure, 14:119-128, 2006

PubMed Abstract: Eukaryotic and archaeal initiation factors 2 (e/aIF2) are heterotrimeric proteins (alphabetagamma) supplying the small subunit of the ribosome with methionylated initiator tRNA. This study reports the crystallographic structure of an aIF2alphagamma heterodimer from Sulfolobus solfataricus bound to Gpp(NH)p-Mg(2+). aIF2gamma is in a closed conformation with the G domain packed on domains II and III. The C-terminal domain of aIF2alpha interacts with domain II of aIF2gamma. Conformations of the two switch regions involved in GTP binding are similar to those encountered in an EF1A:GTP:Phe-tRNA(Phe) complex. Comparison with the EF1A structure suggests that only the gamma subunit of the aIF2alphagamma heterodimer contacts tRNA. Because the alpha subunit markedly reinforces the affinity of tRNA for the gamma subunit, a contribution of the alpha subunit to the switch movements observed in the gamma structure is considered.

PubMed: 16407071
DOI: 10.1016/j.str.2005.09.020

実験手法

X-RAY DIFFRACTION (3 Å)