2AHJ

NITRILE HYDRATASE COMPLEXED WITH NITRIC OXIDE

2AHJ の概要

• エントリーDOI: 10.2210/pdb2ahj/pdb
• 分子名称: NITRILE HYDRATASE, FE (III) ION, ZINC ION, ... (8 entities in total)
• 機能のキーワード: lyase, photoreactive enzyme, nitric oxide binding enzyme, non-heme iron center, post-translational modification of cysteine residues, hydratase
• 由来する生物種: Rhodococcus erythropolis; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 93663.27

構造登録者

Nagashima, S.,Nakasako, M.,Dohmae, N.,Tsujimura, M.,Takio, K.,Odaka, M.,Yohda, M.,Kamiya, N.,Endo, I. (登録日: 1997-12-24, 公開日: 1999-01-27, 最終更新日: 2024-11-20)

主引用文献

Nagashima, S.,Nakasako, M.,Dohmae, N.,Tsujimura, M.,Takio, K.,Odaka, M.,Yohda, M.,Kamiya, N.,Endo, I.
Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms.
Nat.Struct.Biol., 5:347-351, 1998

PubMed Abstract: The iron-containing nitrile hydratase (NHase) is a photoreactive enzyme that is inactivated in the dark because of persistent association with NO and activated by photo-dissociation of NO. The crystal structure at 1.7 A resolution and mass spectrometry revealed the structure of the non-heme iron catalytic center in the nitrosylated state. Two Cys residues coordinated to the iron were post-translationally modified to Cys-sulfenic and -sulfinic acids. Together with another oxygen atom of the Ser ligand, these modifications induced a claw setting of oxygen atoms capturing an NO molecule. This unprecedented structure is likely to enable the photo-regulation of NHase and will provide an excellent model for designing photo-controllable chelate complexes and, ultimately, proteins.

PubMed: 9586994
DOI: 10.1038/nsb0598-347

実験手法

X-RAY DIFFRACTION (1.7 Å)