2AFP

THE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE PROTEIN REVEALS A NEW MEMBER OF THE LECTIN FAMILY

2AFP の概要

• エントリーDOI: 10.2210/pdb2afp/pdb
• NMR情報: BMRB: 4452
• 分子名称: PROTEIN (SEA RAVEN TYPE II ANTIFREEZE PROTEIN) (1 entity in total)
• 機能のキーワード: recombinant sea raven protein, solution backbone fold, c-type lectin, antifreeze protein
• 由来する生物種: Hemitripterus americanus (sea raven)
• 細胞内の位置: Secreted: P05140
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14002.84

構造登録者

Gronwald, W.,Loewen, M.C.,Lix, B.,Daugulis, A.J.,Sonnichsen, F.D.,Davies, P.L.,Sykes, B.D. (登録日: 1998-12-14, 公開日: 1998-12-23, 最終更新日: 2024-10-09)

主引用文献

Gronwald, W.,Loewen, M.C.,Lix, B.,Daugulis, A.J.,Sonnichsen, F.D.,Davies, P.L.,Sykes, B.D.
The solution structure of type II antifreeze protein reveals a new member of the lectin family.
Biochemistry, 37:4712-4721, 1998

PubMed Abstract: A recombinant form of the sea raven type II antifreeze protein (SRAFP) has been produced using the Pichia pastoris expression system. The antifreeze activity of recombinant SRAFP is indistinguishable from that of the wild-type protein. The global fold of SRAFP has been determined by two-dimensional 1H homonuclear and three-dimensional 1H-¿15N¿ heteronuclear NMR spectroscopy using 785 NOE distance restraints and 47 angular restraints. The molecule folds into one globular domain that consists of two helices and nine beta-strands in two beta-sheets. The structure confirms the proposed existence of five disulfide bonds. The global fold of SRAFP is homologous to C-type lectins and pancreatic stone proteins, even though the sequence identity is only approximately 20%.

PubMed: 9537986
DOI: 10.1021/bi972788c

実験手法

SOLUTION NMR