2AFJ

SPRY domain-containing SOCS box protein 2 (SSB-2)

2AFJ の概要

• エントリーDOI: 10.2210/pdb2afj/pdb
• NMR情報: BMRB: 6311
• 分子名称: gene rich cluster, C9 gene (1 entity in total)
• 機能のキーワード: beta sandwich, gene regulation
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Cytoplasm (Probable): O88838
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24891.84

構造登録者

Masters, S.L.,Yao, S.,Willson, T.A.,Zhang, J.G.,Palmer, K.R.,Smith, B.J.,Babon, J.J.,Nicola, N.A.,Norton, R.S.,Nicholson, S.E. (登録日: 2005-07-26, 公開日: 2006-01-03, 最終更新日: 2024-05-29)

主引用文献

Masters, S.L.,Yao, S.,Willson, T.A.,Zhang, J.G.,Palmer, K.R.,Smith, B.J.,Babon, J.J.,Nicola, N.A.,Norton, R.S.,Nicholson, S.E.
The SPRY domain of SSB-2 adopts a novel fold that presents conserved Par-4-binding residues
Nat.Struct.Mol.Biol., 13:77-84, 2006

PubMed Abstract: The four mammalian SPRY domain-containing SOCS box proteins (SSB-1 to SSB-4) are characterized by a C-terminal SOCS box and a central SPRY domain. We have determined the first SPRY-domain structure, as part of SSB-2, by NMR. This domain adopts a novel fold consisting of a beta-sandwich structure formed by two four-stranded antiparallel beta-sheets with a unique topology. We demonstrate that SSB-1, SSB-2 and SSB-4, but not SSB-3, bind prostate apoptosis response protein-4 (Par-4). Mutational analysis of SSB-2 loop regions identified conserved structural determinants for its interaction with Par-4 and the hepatocyte growth factor receptor, c-Met. Mutations in analogous loop regions of pyrin and midline-1 SPRY domains have been shown to cause Mediterranean fever and Opitz syndrome, respectively. Our findings provide a template for SPRY-domain structure and an insight into the mechanism of SPRY-protein interaction.

PubMed: 16369487
DOI: 10.1038/nsmb1034

実験手法

SOLUTION NMR