2ADN

Solution structure of the bacterial antitoxin CcdA: Implications for DNA and toxin binding

2ADN の概要

• エントリーDOI: 10.2210/pdb2adn/pdb
• 関連するPDBエントリー: 2ADL
• NMR情報: BMRB: 6743
• 分子名称: CcdA (1 entity in total)
• 機能のキーワード: ribbon-helix-helix, dna binding protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 16820.86

構造登録者

Madl, T.,VanMelderen, L.,Oberer, M.,Keller, W.,Khatai, L.,Zangger, K. (登録日: 2005-07-20, 公開日: 2006-08-22, 最終更新日: 2024-05-29)

主引用文献

Madl, T.,Van Melderen, L.,Mine, N.,Respondek, M.,Oberer, M.,Keller, W.,Khatai, L.,Zangger, K.
Structural basis for nucleic acid and toxin recognition of the bacterial antitoxin CcdA
J.Mol.Biol., 364:170-185, 2006

PubMed Abstract: Toxin-antitoxin systems are highly abundant in plasmids and bacterial chromosomes. They ensure plasmid maintenance by killing bacteria that have lost the plasmid. Their expression is autoregulated at the level of transcription. Here, we present the solution structure of CcdA, the antitoxin of the ccd system, as a free protein (16.7 kDa) and in complex with its cognate DNA (25.3 kDa). CcdA is composed of two distinct and independent domains: the N-terminal domain, responsible for DNA binding, which establishes a new family of the ribbon-helix-helix fold and the C-terminal region, which is responsible for the interaction with the toxin CcdB. The C-terminal domain is intrinsically unstructured and forms a tight complex with the toxin. We show that CcdA specifically recognizes a 6 bp palindromic DNA sequence within the operator-promoter (OP) region of the ccd operon and binds to DNA by insertion of the positively charged N-terminal beta-sheet into the major groove. The binding of up to three CcdA dimers to a 33mer DNA of its operator-promoter region was studied by NMR spectroscopy, isothermal titration calorimetry and single point mutation. The highly flexible C-terminal region of free CcdA explains its susceptibility to proteolysis by the Lon ATP-dependent protease.

PubMed: 17007877
DOI: 10.1016/j.jmb.2006.08.082

実験手法

SOLUTION NMR