2A7X

Crystal Structure of A Pantothenate synthetase complexed with AMP

2A7X の概要

• エントリーDOI: 10.2210/pdb2a7x/pdb
• 関連するPDBエントリー: 1MOP 1N2B 1N2G 1N2H 1N2J
• 分子名称: Pantoate-beta-alanine ligase, SULFATE ION, ADENOSINE MONOPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: protein-product inhibitor complex, structural genomics, psi, protein structure initiative, tb structural genomics consortium, tbsgc, ligase
• 由来する生物種: Mycobacterium tuberculosis
• 細胞内の位置: Cytoplasm (Potential): P0A5R0
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32127.57

構造登録者

Wang, S.,Eisenberg, D.,TB Structural Genomics Consortium (TBSGC) (登録日: 2005-07-06, 公開日: 2006-02-21, 最終更新日: 2023-08-23)

主引用文献

Wang, S.,Eisenberg, D.
Crystal Structure of the Pantothenate Synthetase from Mycobacterium tuberculosis, Snapshots of the Enzyme in Action.
Biochemistry, 45:1554-1561, 2006

PubMed Abstract: Pantothenate synthetase (PS) from Mycobacterium tuberculosis represents a potential target for antituberculosis drugs. PS catalyzes the ATP-dependent condensation of pantoate and beta-alanine to form pantothenate. Previously, we determined the crystal structure of PS from M. tuberculosis and its complexes with AMPCPP, pantoate, and pantoyl adenylate. Here, we describe the crystal structure of this enzyme complexed with AMP and its last substrate, beta-alanine, and show that the phosphate group of AMP serves as an anchor for the binding of beta-alanine. This structure confirms that binding of beta-alanine in the active site cavity can occur only after formation of the pantoyl adenylate intermediate. A new crystal form was also obtained; it displays the flexible wall of the active site cavity in a conformation incapable of binding pantoate. Soaking of this crystal form with ATP and pantoate gives a fully occupied complex of PS with ATP. Crystal structures of these complexes with substrates, the reaction intermediate, and the reaction product AMP provide a step-by-step view of the PS-catalyzed reaction. A detailed reaction mechanism and its implications for inhibitor design are discussed.

PubMed: 16460002
DOI: 10.1021/bi051873e

実験手法

X-RAY DIFFRACTION (1.7 Å)