2A5I

Crystal structures of SARS coronavirus main peptidase inhibited by an aza-peptide epoxide in the space group C2

2A5I の概要

• エントリーDOI: 10.2210/pdb2a5i/pdb
• 関連するPDBエントリー: 2A5A 2A5K
• 分子名称: 3C-like peptidase, (5S,8S,14R)-ETHYL 11-(3-AMINO-3-OXOPROPYL)-8-BENZYL-14-HYDROXY-5-ISOBUTYL-3,6,9,12-TETRAOXO-1-PHENYL-2-OXA-4,7,10,11-TETRAAZAPENTADECAN-15-OATE, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: cysteine peptidase, 3c-like, n-finger, chymotrypsin-like fold, long loop, alpha-helical domain, dimer, catalytic dyad, specificity pockets, aza-peptide epoxide, substrate-like inhibitor, c-s covalent bond, epoxide stereochemistry, hydrolase
• 由来する生物種: SARS coronavirus
• 細胞内の位置: Non-structural protein 3: Host membrane; Multi-pass membrane protein (Potential). Non-structural protein 4: Host membrane; Multi-pass membrane protein (Potential). Non-structural protein 6: Host membrane; Multi-pass membrane protein (Potential). Non-structural protein 7: Host cytoplasm, host perinuclear region (By similarity). Non-structural protein 8: Host cytoplasm, host perinuclear region (By similarity). Non-structural protein 9: Host cytoplasm, host perinuclear region (By similarity). Non-structural protein 10: Host cytoplasm, host perinuclear region (By similarity). Helicase: Host endoplasmic reticulum-Golgi intermediate compartment (Potential). Uridylate-specific endoribonuclease: Host cytoplasm, host perinuclear region (By similarity): P59641
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34672.51

構造登録者

Lee, T.-W.,Cherney, M.M.,Huitema, C.,Liu, J.,James, K.E.,Powers, J.C.,Eltis, L.D.,James, M.N. (登録日: 2005-06-30, 公開日: 2005-10-25, 最終更新日: 2024-11-13)

主引用文献

Lee, T.-W.,Cherney, M.M.,Huitema, C.,Liu, J.,James, K.E.,Powers, J.C.,Eltis, L.D.,James, M.N.
Crystal Structures of the Main Peptidase from the SARS Coronavirus Inhibited by a Substrate-like Aza-peptide Epoxide
J.Mol.Biol., 353:1137-1151, 2005

PubMed Abstract: The main peptidase (M(pro)) from the coronavirus (CoV) causing severe acute respiratory syndrome (SARS) is one of the most attractive molecular targets for the development of anti-SARS agents. We report the irreversible inhibition of SARS-CoV M(pro) by an aza-peptide epoxide (APE; k(inact)/K(i) = 1900(+/-400) M(-1) s(-1)). The crystal structures of the M(pro):APE complex in the space groups C2 and P2(1)2(1)2(1) revealed the formation of a covalent bond between the catalytic Cys145 S(gamma) atom of the peptidase and the epoxide C3 atom of the inhibitor, substantiating the mode of action of this class of cysteine-peptidase inhibitors. The aza-peptide component of APE binds in the substrate-binding regions of M(pro) in a substrate-like manner, with excellent structural and chemical complementarity. In addition, the crystal structure of unbound M(pro) in the space group C2 revealed that the "N-fingers" (N-terminal residues 1 to 7) of both protomers of M(pro) are well defined and the substrate-binding regions of both protomers are in the catalytically competent conformation at the crystallization pH of 6.5, contrary to the previously determined crystal structures of unbound M(pro) in the space group P2(1).

PubMed: 16219322
DOI: 10.1016/j.jmb.2005.09.004

実験手法

X-RAY DIFFRACTION (1.88 Å)