2A57

Structure of 6,7-Dimthyl-8-ribityllumazine synthase from Schizosaccharomyces pombe mutant W27Y with bound ligand 6-carboxyethyl-7-oxo-8-ribityllumazine

2A57 の概要

• エントリーDOI: 10.2210/pdb2a57/pdb
• 関連するPDBエントリー: 1KYV 1KYX
• 分子名称: 6,7-dimethyl-8-ribityllumazine synthase, 3-[8-((2S,3S,4R)-2,3,4,5-TETRAHYDROXYPENTYL)-2,4,7-TRIOXO-1,3,8-TRIHYDROPTERIDIN-6-YL]PROPANOIC ACID, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: 6, 7-dimethyl-8-ribityllumazine synthase, riboflavin, riboflavin biosynthesis, lusy, transferase
• 由来する生物種: Schizosaccharomyces pombe (fission yeast)
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 88325.21

構造登録者

Koch, M.,Breithaupt, C.,Gerhardt, S.,Haase, I.,Weber, S.,Cushman, M.,Huber, R.,Bacher, A.,Fischer, M. (登録日: 2005-06-30, 公開日: 2005-07-19, 最終更新日: 2023-08-23)

主引用文献

Koch, M.,Breithaupt, C.,Gerhardt, S.,Haase, I.,Weber, S.,Cushman, M.,Huber, R.,Bacher, A.,Fischer, M.
Structural basis of charge transfer complex formation by riboflavin bound to 6,7-dimethyl-8-ribityllumazine synthase
Eur.J.Biochem., 271:3208-3214, 2004

PubMed Abstract: The amino acid residue tryptophan 27 of 6,7-dimethyl-8-ribityllumazine synthase of the yeast Schizosaccharomyces pombe was replaced by tyrosine. The structures of the W27Y mutant protein in complex with riboflavin, the substrate analogue 5-nitroso-6-ribitylamino-2,4(1H,3H)-pyrimidinedione, and the product analogue 6-carboxyethyl-7-oxo-8-ribityllumazine, were determined by X-ray crystallography at resolutions of 2.7-2.8 A. Whereas the indole system of W27 forms a coplanar pi-complex with riboflavin, the corresponding phenyl ring in the W27Y mutant establishes only peripheral contact with the heterocyclic ring system of the bound riboflavin. These findings provide an explanation for the absence of the long wavelength shift in optical absorption spectra of riboflavin bound to the mutant enzyme. The structures of the mutants are important tools for the interpretation of the unusual physical properties of riboflavin in complex with lumazine synthase.

PubMed: 15265040
DOI: 10.1111/j.1432-1033.2004.04253.x

実験手法

X-RAY DIFFRACTION (2.75 Å)