2A55

Solution structure of the two N-terminal CCP modules of C4b-binding protein (C4BP) alpha-chain.

2A55 の概要

• エントリーDOI: 10.2210/pdb2a55/pdb
• 分子名称: C4b-binding protein (1 entity in total)
• 機能のキーワード: complement, scr, ccp module, immune system
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P04003
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15067.94

構造登録者

Jenkins, H.T.,Mark, L.,Ball, G.,Lindahl, G.,Uhrin, D.,Blom, A.M.,Barlow, P.N. (登録日: 2005-06-30, 公開日: 2005-12-13, 最終更新日: 2024-11-20)

主引用文献

Jenkins, H.T.,Mark, L.,Ball, G.,Persson, J.,Lindahl, G.,Uhrin, D.,Blom, A.M.,Barlow, P.N.
Human C4b-binding Protein, Structural Basis for Interaction with Streptococcal M Protein, a Major Bacterial Virulence Factor
J.Biol.Chem., 281:3690-3697, 2006

PubMed Abstract: Human C4b-binding protein (C4BP) protects host tissue, and those pathogens able to hijack this plasma glycoprotein, from complement-mediated destruction. We now show that the first two complement control protein (CCP) modules of the C4BP alpha-chain, plus the four residues connecting them, are necessary and sufficient for binding a bacterial virulence factor, the Streptococcus pyogenes M4 (Arp4) protein. Structure determination by NMR reveals two tightly coupled CCP modules in an elongated arrangement within this region of C4BP. Chemical shift perturbation studies demonstrate that the N-terminal, hypervariable region of M4 binds to a site including strand 1 of CCP module 2. This interaction is accompanied by an intermodular reorientation within C4BP. We thus provide a detailed picture of an interaction whereby a pathogen evades complement.

PubMed: 16330538
DOI: 10.1074/jbc.M511563200

実験手法

SOLUTION NMR