2A3D

SOLUTION STRUCTURE OF A DE NOVO DESIGNED SINGLE CHAIN THREE-HELIX BUNDLE (A3D)

2A3D の概要

• エントリーDOI: 10.2210/pdb2a3d/pdb
• 分子名称: PROTEIN (DE NOVO THREE-HELIX BUNDLE) (1 entity in total)
• 機能のキーワード: three-helix bundle
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8120.13

構造登録者

Walsh, S.T.R.,Cheng, H.,Bryson, J.W.,Roder, H.,Degrado, W.F. (登録日: 1999-04-01, 公開日: 1999-05-05, 最終更新日: 2023-12-27)

主引用文献

Walsh, S.T.,Cheng, H.,Bryson, J.W.,Roder, H.,DeGrado, W.F.
Solution structure and dynamics of a de novo designed three-helix bundle protein.
Proc.Natl.Acad.Sci.USA, 96:5486-5491, 1999

PubMed Abstract: Although de novo protein design is an important endeavor with implications for understanding protein folding, until now, structures have been determined for only a few 25- to 30-residue designed miniproteins. Here, the NMR solution structure of a complex 73-residue three-helix bundle protein, alpha3D, is reported. The structure of alpha3D was not based on any natural protein, and yet it shows thermodynamic and spectroscopic properties typical of native proteins. A variety of features contribute to its unique structure, including electrostatics, the packing of a diverse set of hydrophobic side chains, and a loop that incorporates common capping motifs. Thus, it is now possible to design a complex protein with a well defined and predictable three-dimensional structure.

PubMed: 10318910
DOI: 10.1073/pnas.96.10.5486

実験手法

SOLUTION NMR