2A0X

Structure of human purine nucleoside phosphorylase H257F mutant

2A0X の概要

• エントリーDOI: 10.2210/pdb2a0x/pdb
• 関連するPDBエントリー: 1A0W 2A0Y
• 分子名称: Purine nucleoside phosphorylase, SULFATE ION, 7-[[(3R,4R)-3-(hydroxymethyl)-4-oxidanyl-pyrrolidin-1-ium-1-yl]methyl]-3,5-dihydropyrrolo[3,2-d]pyrimidin-4-one, ... (4 entities in total)
• 機能のキーワード: purine nucleoside phosphorylase, transition state inhibitor, mutant, transferase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32651.32

構造登録者

Murkin, A.S.,Shi, W.,Schramm, V.L. (登録日: 2005-06-17, 公開日: 2006-06-06, 最終更新日: 2023-08-23)

主引用文献

Murkin, A.S.,Birck, M.R.,Rinaldo-Matthis, A.,Shi, W.,Taylor, E.A.,Almo, S.C.,Schramm, V.L.
Neighboring group participation in the transition state of human purine nucleoside phosphorylase.
Biochemistry, 46:5038-5049, 2007

PubMed Abstract: The X-ray crystal structures of human purine nucleoside phosphorylase (PNP) with bound inosine or transition-state analogues show His257 within hydrogen bonding distance of the 5'-hydroxyl. The mutants His257Phe, His257Gly, and His257Asp exhibited greatly decreased affinity for Immucillin-H (ImmH), binding this mimic of an early transition state as much as 370-fold (Km/Ki) less tightly than native PNP. In contrast, these mutants bound DADMe-ImmH, a mimic of a late transition state, nearly as well as the native enzyme. These results indicate that His257 serves an important role in the early stages of transition-state formation. Whereas mutation of His257 resulted in little variation in the PNP x DADMe-ImmH x SO4 structures, His257Phe x ImmH x PO4 showed distortion at the 5'-hydroxyl, indicating the importance of H-bonding in positioning this group during progression to the transition state. Binding isotope effect (BIE) and kinetic isotope effect (KIE) studies of the remote 5'-(3)H for the arsenolysis of inosine with native PNP revealed a BIE of 1.5% and an unexpectedly large intrinsic KIE of 4.6%. This result is interpreted as a moderate electronic distortion toward the transition state in the Michaelis complex with continued development of a similar distortion at the transition state. The mutants His257Phe, His257Gly, and His257Asp altered the 5'-(3)H intrinsic KIE to -3, -14, and 7%, respectively, while the BIEs contributed 2, 2, and -2%, respectively. These surprising results establish that forces in the Michaelis complex, reported by the BIEs, can be reversed or enhanced at the transition state.

PubMed: 17407325
DOI: 10.1021/bi700147b

実験手法

X-RAY DIFFRACTION (2.28 Å)