29ST

Azetidine-1- carboxylic acid synthase AzeJ from Saccharotrix sp. NRRL B-16348

29ST の概要

• エントリーDOI: 10.2210/pdb29st/pdb
• 分子名称: Methyltransferase family protein (2 entities in total)
• 機能のキーワード: azej, azetidine-1- carboxylic acid, sam, sah, methyl-transferase, natural products, lyase
• 由来する生物種: Saccharothrix sp. NRRL B-16348
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 135098.60

構造登録者

Lukat, P.,Oral, G.,Blankenfeldt, W. (登録日: 2026-04-07, 公開日: 2026-08-05)

主引用文献

Germer, P.,Gericke, L.,Koeppl, L.H.,Zou, Z.,Jockmann, E.,Kuge, M.,Zoller, K.,Herrmann, H.,Fuderer, R.,Mohr, M.K.F.,Bartels, A.,Oral, G.,Lukat, P.,Layer, G.,Muller, M.,Blankenfeldt, W.,Barra, L.,Andexer, J.N.
S-Adenosyl-d-Methionine as a Non-Physiological Substrate for a Wide Range of SAM-Dependent Enzymes.
Chembiochem, 27:e70467-e70467, 2026

PubMed Abstract: The ability of SAM-dependent enzymes to accept S-adenosyl-d-methionine [d-SAM, (S,R)-SAM] instead of the native cofactor S-adenosyl-l-methionine [l-SAM, (S,S)-SAM] remains largely unexplored. Challenging the stereochemical preference of SAM-dependent enzymes, we investigated the ability of different enzyme classes to accept d-SAM. Contrary to common assumptions, the tested N- and O-methyltransferases (MTs), as well as one of the examined C-MTs accepted d-SAM. Docking studies suggest that acceptance of d-SAM by C-MTs may be influenced by the angle between the transferable methyl group of SAM and the nucleophilic carbon of the substrate, along with enzyme and substrate flexibility. In addition to conventional MTs, the radical SAM glutamine C-MT QCMT showed low but detectable methylation activity with d-SAM. Furthermore, the azetidine-2-carboxylic acid synthase AzeJ not only uses d-SAM but also incorporates the stereocentre of d-methionine into the cyclic amino acid product. The pyridoxal 5'-phosphate (PLP)-dependent enzyme 1-aminocyclopropyl-1-carboxylic acid synthase (ACCS) also showed detectable turnover with d-SAM. These findings broaden the understanding of enzyme stereoselectivity, provide an overview of d-SAM-utilising enzymes, and identify the first enzyme systems that may serve as starting points for engineering efforts aimed at shifting cofactor preference towards d-SAM.

PubMed: 42503187
DOI: 10.1002/cbic.70467

実験手法

X-RAY DIFFRACTION (2.67 Å)