299D

CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: THE HAMMERHEAD RIBOZYME

299D の概要

• エントリーDOI: 10.2210/pdb299d/pdb
• 分子名称: RNA HAMMERHEAD RIBOZYME (2 entities in total)
• 機能のキーワード: rna hammerhead ribozyme, catalytic rna, loop, ribozyme
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13189.98

構造登録者

Scott, W.G.,Murray, J.B.,Arnold, J.R.P.,Stoddard, B.L.,Klug, A. (登録日: 1996-12-14, 公開日: 1997-01-24, 最終更新日: 2024-02-14)

主引用文献

Scott, W.G.,Murray, J.B.,Arnold, J.R.,Stoddard, B.L.,Klug, A.
Capturing the structure of a catalytic RNA intermediate: the hammerhead ribozyme.
Science, 274:2065-2069, 1996

PubMed Abstract: The crystal structure of an unmodified hammerhead RNA in the absence of divalent metal ions has been solved, and it was shown that this ribozyme can cleave itself in the crystal when divalent metal ions are added. This biologically active RNA fold is the same as that found previously for two modified hammerhead ribozymes. Addition of divalent cations at low pH makes it possible to capture the uncleaved RNA in metal-bound form. A conformational intermediate, having an additional Mg(II) bound to the cleavage-site phosphate, was captured by freeze-trapping the RNA at an active pH prior to cleavage. The most significant conformational changes were limited to the active site of the ribozyme, and the changed conformation requires only small additional movements to reach a proposed transition-state.

PubMed: 8953035
DOI: 10.1126/science.274.5295.2065

実験手法

X-RAY DIFFRACTION (3 Å)