293D

INTERACTION BETWEEN THE LEFT-HANDED Z-DNA AND POLYAMINE-2: THE CRYSTAL STRUCTURE OF THE D(CG)3 AND SPERMIDINE COMPLEX

293D の概要

• エントリーDOI: 10.2210/pdb293d/pdb
• 分子名称: DNA (5'-D(*CP*GP*CP*GP*CP*G)-3'), SPERMIDINE, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: z-dna, double helix, complexed with spermidine, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 3861.56

構造登録者

Ohishi, H.,Nakanishi, I.,Inubushi, K.,Van Der Marel, G.A.,Van Boom, J.H.,Rich, A.,Wang, A.H.-J.,Hakoshima, T.,Tomita, K. (登録日: 1996-10-09, 公開日: 1996-12-02, 最終更新日: 2024-04-03)

主引用文献

Ohishi, H.,Nakanishi, I.,Inubushi, K.,van der Marel, G.,van Boom, J.H.,Rich, A.,Wang, A.H.,Hakoshima, T.,Tomita, K.
Interaction between the left-handed Z-DNA and polyamine-2. The crystal structure of the d(CG)3 and spermidine complex.
FEBS Lett., 391:153-156, 1996

PubMed Abstract: This paper deals with the crystal structure of d(CG)3-spermidine complex. The DNA fragment, d(CG)3, was crystallized with N-(2-amino-propyl)-1,4-diamino-butane, PA(34), spermidine. The results of its X-ray crystallographic analysis showed many intermolecular contacts between d(CG)3 and spermidine, but the binding mode of spermidine to the d(CG)3 molecule is different from that of the d(CG)3 and N-(2-amino-ethyl)-1,4-diamino-butane [PA(24)] complex: a spermidine molecule bound to the d(CG)3 and its symmetrically related neighboring d(CG)3 molecules through the water molecules with hydrogen bonds, while one PA(24) molecule connected directly to one d(CG)3 molecule, but not to its neighboring d(CG)3 molecule. In the crystal, the d(CG)3 molecule was the left-handed Z-form, and three magnesium cations and a sodium cation were observed around the d(CG)3 moiety with different binding modes from the case of the d(CG)3-PA(24) complex.

PubMed: 8706905
DOI: 10.1016/0014-5793(96)00723-5

実験手法

X-RAY DIFFRACTION (1 Å)