28SR

NMR STRUCTURE OF THE MOST CONSERVED RNA MOTIF IN SRP RNA

28SR の概要

• エントリーDOI: 10.2210/pdb28sr/pdb
• 分子名称: SRP DOMAIN IV (1 entity in total)
• 機能のキーワード: nmr of rna, srp, 4.5s rna, rna structure, complete relaxation matrix analysis, rna
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9126.53

構造登録者

Schmitz, U.,James, T.L.,Lukavsky, P.,Walter, P. (登録日: 1999-04-15, 公開日: 1999-04-20, 最終更新日: 2023-12-27)

主引用文献

Schmitz, U.,James, T.L.,Lukavsky, P.,Walter, P.
Structure of the most conserved internal loop in SRP RNA.
Nat.Struct.Biol., 6:634-638, 1999

PubMed Abstract: The signal recognition particle (SRP) directs translating ribosomes to the protein translocation apparatus of endoplasmic reticulum (ER) membrane or the bacterial plasma membrane. The SRP is universally conserved, and in prokaryotes consists of two essential subunits, SRP RNA and SRP54, the latter of which binds to signal sequences on the nascent protein chains. Here we describe the solution NMR structure of a 28-mer RNA composing the most conserved part of SRP RNA to which SRP54 binds. Central to this function is a six-nucleotide internal loop that assumes a novel Mg2+-dependent structure with unusual cross-strand interactions; besides a cross-strand A/A stack, two guanines form hydrogen bonds with opposite-strand phosphates. The structure completely explains the phylogenetic conservation of the loop bases, underlining its importance for SRP54 binding and SRP function.

PubMed: 10404218
DOI: 10.1038/10683

実験手法

SOLUTION NMR