259D

RNA HYDRATION: A DETAILED LOOK

259D の概要

• エントリーDOI: 10.2210/pdb259d/pdb
• 分子名称: RNA (5'-R(*CP*CP*CP*CP*GP*GP*GP*G)-3') (2 entities in total)
• 機能のキーワード: a-rna, double helix, rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 5113.19

構造登録者

Egli, M.,Portmann, S.,Usman, N. (登録日: 1996-05-03, 公開日: 1996-08-26, 最終更新日: 2024-02-14)

主引用文献

Egli, M.,Portmann, S.,Usman, N.
RNA hydration: a detailed look.
Biochemistry, 35:8489-8494, 1996

PubMed Abstract: The crystal structure of the RNA duplex [r(CCCCGGGG)]2 has been refined to 1.46 A resolution with room temperature synchrotron diffraction data. This represents the highest resolution reported to date for an all-RNA oligonucleotide and is well beyond the best resolution ever achieved with an A-form DNA duplex. The analysis of the ordered hydration around the octamer duplex reveals conserved regular arrangements of water molecules in both grooves. In the major groove, all located first shell water molecules can be fitted into a pattern that is repeated through all eight base pairs, involves half the phosphate oxygens, and joins the two strands. In the minor groove, roughly across its narrowest dimension, tandem water molecules link the 2'-hydroxyl groups of adjacent nucleotides in base-pair steps in a similarly regular fashion. The structure provides evidence for an important role of the 2'-hydroxyl groups in the thermodynamic stabilization of RNA, beyond their known functions of locking the sugar pucker and mediating 3' --> 5' intrastrand O2'...O4' hydrogen bonds. The ribose 2'-hydroxyls lay the foundation for the enthalpic stability of the RNA relative to the DNA duplex, both as a scaffold for the water network in the minor groove and through their extensive individual hydration.

PubMed: 8679609
DOI: 10.1021/bi9607214

実験手法

X-RAY DIFFRACTION (1.46 Å)