233D

THE CRYSTAL STRUCTURE ANALYSIS OF D(CGCGAASSCGCG)2: A SYNTHETIC DNA DODECAMER DUPLEX CONTAINING FOUR 4'-THIO-2'-DEOXYTHYMIDINE NUCLEOTIDES

233D の概要

• エントリーDOI: 10.2210/pdb233d/pdb
• 分子名称: DNA (5'-D(*CP*GP*CP*GP*AP*AP*)-D(*(T49)P*(T49)P*)-D(*CP*GP*CP*G)-3') (2 entities in total)
• 機能のキーワード: b-dna, double helix, modified, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7391.05

構造登録者

Boggon, T.J.,Hancox, E.L.,McAuley-Hecht, K.E.,Connolly, B.A.,Hunter, W.N.,Brown, T.,Walker, R.T.,Leonard, G.A. (登録日: 1995-10-16, 公開日: 1996-06-21, 最終更新日: 2024-02-14)

主引用文献

Boggon, T.J.,Hancox, E.L.,McAuley-Hecht, K.E.,Connolly, B.A.,Hunter, W.N.,Brown, T.,Walker, R.T.,Leonard, G.A.
The crystal structure analysis of d(CGCGAASSCGCG)2, a synthetic DNA dodecamer duplex containing four 4'-thio-2'-deoxythymidine nucleotides.
Nucleic Acids Res., 24:951-961, 1996

PubMed Abstract: The crystal structure refinement of the synthetic dodecamer d(CGCGAASSCGCG), where S = 4'-thio-2'-deoxythymidine, has converged at R=0.201 for 2605 reflections with F > 2sigma(F) in the resolution range 8.0-2.4 A for a model consisting of the dodecamer duplex and 66 water molecules. A comparison of its structure with that of the native dodecamer d(CGCGAATTCGCG) has revealed that the major differences between the two structures is a change in the conformation of the sugar-phosphate backbone in the regions at and adjacent to the positions of the modified nucleosides. Examination of the fine structural parameters for each of the structures reveals that the thiosugars adopt a C3'-exo conformation in d(CGCGAASSCGCG), rather than the approximate C1'-exo conformation found for the analogous sugars in the structure of d(CGCGAATTCGCG). The observed differences in structure between the two duplexes may help to explain the enhanced resistance to nuclease digestion of synthetic oligonucleotides containing 4'-thio-2'-deoxynucleotides.

PubMed: 8600465
DOI: 10.1093/nar/24.5.951

実験手法

X-RAY DIFFRACTION (2.4 Å)