225D

A TETRAMERIC DNA STRUCTURE WITH PROTONATED CYTOSINE:CYTOSINE BASE PAIRS

225D の概要

• エントリーDOI: 10.2210/pdb225d/pdb
• 分子名称: DNA (5'-D(*TP*CP*CP*CP*CP*C)-3') (1 entity in total)
• 機能のキーワード: dna, intercalated cytosine tetrad
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 6820.58

構造登録者

Gehring, K.,Leroy, J.L.,Gueron, M. (登録日: 1995-07-12, 公開日: 1995-10-15, 最終更新日: 2024-05-22)

主引用文献

Gehring, K.,Leroy, J.L.,Gueron, M.
A tetrameric DNA structure with protonated cytosine.cytosine base pairs.
Nature, 363:561-565, 1993

PubMed Abstract: Oligomers containing tracts of cytidine form hemiprotonated base pairs at acid pH and have been considered to be double-stranded. We have solved the structure of the DNA oligomer 5'-d(TCCCCC) at acid pH and find that it is a four-stranded complex in which two base-paired parallel-stranded duplexes are intimately associated, with their base pairs fully intercalated. The relative orientation of the duplexes is antiparallel, so that each base pair is face-to-face with its neighbours. The NMR spectrum displays only six spin systems, showing that the structure is highly symmetrical on the NMR timescale; the four strands are equivalent. A model derived by energy minimization and constrained molecular dynamics shows excellent compatibility with the observed nuclear Overhauser effects (NOEs) particularly for the very unusual inter-residue sugar-sugar NOEs H1'-H1', H1'-H2" and H1'-H4'. These NOEs are probably diagnostic for such tetrameric structures.

PubMed: 8389423
DOI: 10.1038/363561a0

実験手法

SOLUTION NMR