201D

SOLUTION STRUCTURE OF THE OXYTRICHA TELOMERIC REPEAT D[G4(T4G4)3] G-TETRAPLEX

201D の概要

• エントリーDOI: 10.2210/pdb201d/pdb
• 分子名称: INTRAMOLECULAR G-TETRAPLEX DNA (1 entity in total)
• 機能のキーワード: dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8872.65

構造登録者

Wang, Y.,Patel, D.J. (登録日: 1995-03-16, 公開日: 1995-07-31, 最終更新日: 2024-05-22)

主引用文献

Wang, Y.,Patel, D.J.
Solution structure of the Oxytricha telomeric repeat d[G4(T4G4)3] G-tetraplex.
J.Mol.Biol., 251:76-94, 1995

PubMed Abstract: The solution structure of Oxytricha telomere sequence d[G4(T4G4)3] in 0.1 M Na+ containing solution has been determined using a combined NMR-molecular dynamics approach including relaxation matrix refinement. This four G4 repeat sequence folds intramolecularly into a right-handed G-tetraplex containing four stacked G-tetrads which are connected by two lateral T4 loops and a central diagonal T4 loop. The guanine glycosidic bonds adopt a syn-anti alternation along the full length of the d[G4(T4G4)3] sequence while the orientation around adjacent G-tetrads switches between syn.syn.anti.anti and anti.anti.syn.syn alignments. Four distinct grooves are formed by the parallel (two of medium width) and anti-parallel (one wide and one narrow width) alignment of adjacent G-G-G-G segments in the G-tetraplex. The T4 residues in the diagonal loop are well-defined while the T4 residues in both lateral loops are under-defined and sample multiple conformations. The solution structure of the Na(+)-stabilized Oxytricha d[G4(T4G4)3] G-tetraplex and an earlier solution structure reported from our laboratory on the Na(+)-stabilized human d[AG3(T2AG3)3] G-tetraplex exhibit a common folding topology defined by the same syn/anti distribution of guanine residues along individual strands and around individual G-tetrads, as well as a common central diagonal loop which defines the strand directionalities. The well-resolved proton NMR spectra associated with the d[G4(T4G4)3] G-tetraplex opens the opportunity for studies ranging from cation-dependent characterization of G-tetraplex conformation and hydration to ligand and protein recognition of the distinct grooves associated with this folding topology.

PubMed: 7643391
DOI: 10.1006/jmbi.1995.0417

実験手法

SOLUTION NMR