200D

STABLE LOOP IN THE CRYSTAL STRUCTURE OF THE INTERCALATED FOUR-STRANDED CYTOSINE-RICH METAZOAN TELOMERE

Summary for 200D

• Entry DOI: 10.2210/pdb200d/pdb
• Descriptor: DNA (5'-D(*TP*AP*AP*CP*CP*C)-3') (2 entities in total)
• Functional Keywords: u-dna, quadruple helix, tetraplex, base intercalated, loop, dna
• Total number of polymer chains: 2
• Total formula weight: 3506.39

Authors

Kang, C.,Berger, I.,Lockshin, C.,Ratliff, R.,Moyzis, R.,Rich, A. (deposition date: 1995-02-16, release date: 1995-07-31, Last modification date: 2024-02-14)

Primary citation

Kang, C.,Berger, I.,Lockshin, C.,Ratliff, R.,Moyzis, R.,Rich, A.
Stable loop in the crystal structure of the intercalated four-stranded cytosine-rich metazoan telomere.
Proc.Natl.Acad.Sci.USA, 92:3874-3878, 1995

PubMed Abstract: In most metazoans, the telomeric cytosine-rich strand repeating sequence is d(TAACCC). The crystal structure of this sequence was solved to 1.9-A resolution. Four strands associate via the cytosine-containing parts to form a four-stranded intercalated structure held together by C.C+ hydrogen bonds. The base-paired strands are parallel to each other, and the two duplexes are intercalated into each other in opposite orientations. One TAA end forms a highly stabilized loop with the 5' thymine Hoogsteen-base-paired to the third adenine. The 5' end of this loop is in close proximity to the 3' end of one of the other intercalated cytosine strands. Instead of being entirely in a DNA duplex, this structure suggests the possibility of an alternative conformation for the cytosine-rich telomere strands.

PubMed: 7731999
DOI: 10.1073/pnas.92.9.3874

Experimental method

X-RAY DIFFRACTION (1.85 Å)