1ZZA

Solution NMR Structure of the Membrane Protein Stannin

1ZZA の概要

• エントリーDOI: 10.2210/pdb1zza/pdb
• NMR情報: BMRB: 6715
• 分子名称: Stannin (1 entity in total)
• 機能のキーワード: helix, membrane protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Mitochondrion outer membrane; Single-pass membrane protein: O75324
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9650.21

構造登録者

Buck-Koehntop, B.A.,Mascioni, A.,Buffy, J.J.,Veglia, G. (登録日: 2005-06-13, 公開日: 2005-12-20, 最終更新日: 2024-05-22)

主引用文献

Buck-Koehntop, B.A.,Mascioni, A.,Buffy, J.J.,Veglia, G.
Structure, dynamics, and membrane topology of stannin: a mediator of neuronal cell apoptosis induced by trimethyltin chloride.
J.Mol.Biol., 354:652-665, 2005

PubMed Abstract: Organotin compounds or alkyltins are ubiquitous environmental toxins that have been implicated in cellular death. Unlike other xenobiotic compounds, such as organomercurials and organoleads, alkyltins activate apoptotic cascades at low concentrations. Trimethyltin (TMT) chloride is amongst the most toxic organotin compounds, and is known to selectively inflict injury to specific regions of the brain. Stannin (SNN), an 88-residue mitochondrial membrane protein, has been identified as the specific marker for neuronal cell apoptosis induced by TMT intoxication. This high specificity of TMT makes SNN an ideal model system for understanding the mechanism of organotin neurotoxicity at a molecular level. Here, we report the three-dimensional structure and dynamics of SNN in detergent micelles, and its topological orientation in lipid bilayers as determined by solution and solid-state NMR spectroscopy. We found that SNN is a monotopic membrane protein composed of three domains: a single transmembrane helix (residues 10-33) that transverses the lipid bilayer at approximately a 20 degrees angle with respect to the membrane normal; a 28 residue unstructured linker, which includes a conserved CXC metal-binding motif and a putative 14-3-3zeta binding domain; and a distorted cytoplasmic helix (residues 61-79) that is partially absorbed into the plane of the lipid bilayer with a tilt angle of approximately 80 degrees from the membrane normal. The structure and architecture of SNN within the lipid environment provides insight about how this protein transmits toxic insults caused by TMT across the membrane.

PubMed: 16246365
DOI: 10.1016/j.jmb.2005.09.038

実験手法

SOLUTION NMR