1ZUX

EosFP Fluorescent Protein- Green Form

1ZUX の概要

• エントリーDOI: 10.2210/pdb1zux/pdb
• 関連するPDBエントリー: 2btj
• 分子名称: green to red photoconvertible GPF-like protein EosFP (2 entities in total)
• 機能のキーワード: fluorescent protein, photoconversion, peptide bond cleavage, beta-elimination, luminescent protein
• 由来する生物種: Lobophyllia hemprichii
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 103229.03

構造登録者

Nar, H.,Nienhaus, K.,Wiedenmann, J.,Nienhaus, G.U. (登録日: 2005-06-01, 公開日: 2005-06-28, 最終更新日: 2026-03-18)

主引用文献

Nienhaus, K.,Nienhaus, G.U.,Wiedenmann, J.,Nar, H.
Structural basis for photo-induced protein cleavage and green-to-red conversion of fluorescent protein EosFP.
Proc.Natl.Acad.Sci.Usa, 102:9156-9159, 2005

PubMed Abstract: Genetically encoded fusion constructs derived from fluorescent proteins (FPs) can be designed to report on a multitude of events and signals in cells, tissues, and entire organs without interfering with the complex machinery of life. EosFP is a novel FP from the scleractinian coral Lobophyllia hemprichii that switches its fluorescence emission from green (516 nm) to red (581 nm) upon irradiation with approximately 400-nm light. This property enables localized tagging of proteins and thus provides a valuable tool for tracking protein movements within live cells. Here, we present the x-ray structures of the green and red forms of WT EosFP. They reveal that formation of the red chromophore is associated with cleavage of the peptide backbone, with surprisingly little change elsewhere in the structure, and provide insights into the mechanism that generates this interesting posttranslational polypeptide modification.

PubMed: 15964985
DOI: 10.1073/pnas.0501874102

実験手法

X-RAY DIFFRACTION (1.85 Å)