1ZUE

Revised Solution Structure of DLP-2

1ZUE の概要

• エントリーDOI: 10.2210/pdb1zue/pdb
• 関連するPDBエントリー: 1D6B 1ZUF
• 分子名称: Defensin-like peptide 2/4 (1 entity in total)
• 機能のキーワード: helix, antiparallel beta-sheet, toxin
• 細胞内の位置: Secreted: P82140
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5121.90

構造登録者

Torres, A.M.,Tsampazi, C.,Geraghty, D.P.,Bansal, P.S.,Alewood, P.F.,Kuchel, P.W. (登録日: 2005-05-30, 公開日: 2005-08-02, 最終更新日: 2024-10-30)

主引用文献

Torres, A.M.,Tsampazi, C.,Geraghty, D.P.,Bansal, P.S.,Alewood, P.F.,Kuchel, P.W.
D-amino acid residue in a defensin-like peptide from platypus venom: effect on structure and chromatographic properties.
Biochem.J., 391:215-220, 2005

PubMed Abstract: The recent discovery that the natriuretic peptide OvCNPb (Ornithorhynchus venom C-type natriuretic peptide B) from platypus (Ornithorynchus anatinus) venom contains a D-amino acid residue suggested that other D-amino-acid-containing peptides might be present in the venom. In the present study, we show that DLP-2 (defensin-like peptide-2), a 42-amino-acid residue polypeptide in the platypus venom, also contains a D-amino acid residue, D-methionine, at position 2, while DLP-4, which has an identical amino acid sequence, has all amino acids in the L-form. These findings were supported further by the detection of isomerase activity in the platypus gland venom extract that converts DLP-4 into DLP-2. In the light of this new information, the tertiary structure of DLP-2 was recalculated using a new structural template with D-Met2. The structure of DLP-4 was also determined in order to evaluate the effect of a D-amino acid at position 2 on the structure and possibly to explain the large retention time difference observed for the two molecules in reverse-phase HPLC. The solution structures of the DLP-2 and DLP-4 are very similar to each other and to the earlier reported structure of DLP-2, which assumed that all amino acids were in the L-form. Our results suggest that the incorporation of the D-amino acid at position 2 has minimal effect on the overall fold in solution.

PubMed: 16033333
DOI: 10.1042/BJ20050900

実験手法

SOLUTION NMR