1ZUB

Solution Structure of the RIM1alpha PDZ Domain in Complex with an ELKS1b C-terminal Peptide

1ZUB の概要

• エントリーDOI: 10.2210/pdb1zub/pdb
• 分子名称: Regulating synaptic membrane exocytosis protein 1, ELKS1b (2 entities in total)
• 機能のキーワード: pdz domain, complex, endocytosis-exocytosis complex, endocytosis/exocytosis
• 由来する生物種: Rattus norvegicus (Norway rat); 詳細
• 細胞内の位置: Cell membrane; Peripheral membrane protein: Q9JIR4; Cytoplasm (By similarity): Q811U3
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13538.42

構造登録者

Lu, J.,Li, H.,Wang, Y.,Sudhof, T.C.,Rizo, J. (登録日: 2005-05-30, 公開日: 2005-08-30, 最終更新日: 2024-05-22)

主引用文献

Lu, J.,Li, H.,Wang, Y.,Sudhof, T.C.,Rizo, J.
Solution Structure of the RIM1alpha PDZ Domain in Complex with an ELKS1b C-terminal Peptide
J.Mol.Biol., 352:455-466, 2005

PubMed Abstract: PDZ domains are widespread protein modules that commonly recognize C-terminal sequences of target proteins and help to organize macromolecular signaling complexes. These sequences usually bind in an extended conformation to relatively shallow grooves formed between a beta-strand and an alpha-helix in the corresponding PDZ domains. Because of this binding mode, many PDZ domains recognize primarily the C-terminal and the antepenultimate side-chains of the target protein, which commonly conform to motifs that have been categorized into different classes. However, an increasing number of PDZ domains have been found to exhibit unusual specificities. These include the PDZ domain of RIMs, which are large multidomain proteins that regulate neurotransmitter release and help to organize presynaptic active zones. The RIM PDZ domain binds to the C-terminal sequence of ELKS with a unique specificity that involves each of the four ELKS C-terminal residues. To elucidate the structural basis for this specificity, we have determined the 3D structure in solution of an RIM/ELKS C-terminal peptide complex using NMR spectroscopy. The structure shows that the RIM PDZ domain contains an unusually deep and narrow peptide-binding groove with an exquisite shape complementarity to the four ELKS C-terminal residues in their bound conformation. This groove is formed, in part, by a set of side-chains that is conserved selectively in RIM PDZ domains and that hence determines, at least in part, their unique specificity.

PubMed: 16095618
DOI: 10.1016/j.jmb.2005.07.047

実験手法

SOLUTION NMR