1ZTB

Crystal Structure of Chorismate Synthase from Mycobacterium tuberculosis

1ZTB の概要

• エントリーDOI: 10.2210/pdb1ztb/pdb
• 分子名称: Chorismate synthase (2 entities in total)
• 機能のキーワード: beta-alpha-beta, flavoprotein, shikimate, ligase
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 41844.38

構造登録者

Dias, M.V.B.,Borges, J.C.,Ely, F.,Pereira, J.H.,Canduri, F.,Ramos, C.H.I.,Frazzon, J.,Palma, M.S.,Basso, L.A.,Santos, D.S.,Azevedo Jr., W.F. (登録日: 2005-05-26, 公開日: 2006-05-16, 最終更新日: 2023-08-23)

主引用文献

Dias, M.V.B.,Borges, J.C.,Ely, F.,Pereira, J.H.,Canduri, F.,Ramos, C.H.I.,Frazzon, J.,Palma, M.S.,Basso, L.A.,Santos, D.S.,de Azevedo Jr., W.F.
Structure of chorismate synthase from Mycobacterium tuberculosis
J.Struct.Biol., 154:130-143, 2006

PubMed Abstract: In bacteria, fungi, plants, and apicomplexan parasites, the aromatics compounds, such as aromatics amino acids, are synthesized through seven enzymes from the shikimate pathway, which are absent in mammals. The absence of this pathway in mammals make them potential targets for development of new therapy against infectious diseases, such as tuberculosis, which is the world's second commonest cause of death from infectious disease. The last enzyme of shikimate pathway is the chorismate synthase (CS), which is responsible for conversion of the 5-enolpyruvylshikimate-3-phosphate to chorismate. Here, we report the crystallographic structure of CS from Mycobacterium tuberculosis (MtCS) at 2.65 A resolution. The MtCS structure is similar to other CS structures, presenting beta-alpha-beta sandwich structural topology, in which each monomer of MtCS consists of a central helical core. The MtCS can be described as a tetramer formed by a dimer of dimers. However, analytical ultracentrifugation studies suggest the MtCS is a dimer with a more asymmetric shape than observed on the crystallographic dimer and the existence of a low equilibrium between dimer and tetramer. Our results suggest that the MtCS oligomerization is concentration dependent and some conformational changes must be involved on that event.

PubMed: 16459102
DOI: 10.1016/j.jsb.2005.12.008

実験手法

X-RAY DIFFRACTION (2.65 Å)