1ZM2

Structure of ADP-ribosylated eEF2 in complex with catalytic fragment of ETA

1ZM2 の概要

• エントリーDOI: 10.2210/pdb1zm2/pdb
• 関連するPDBエントリー: 1AER 1U2R 1ZM3 1ZM4 1ZM9
• 分子名称: Elongation factor 2, Exotoxin A, ADENOSINE-5-DIPHOSPHORIBOSE (3 entities in total)
• 機能のキーワード: elongation factor, toxin, adp-ribosylation, biosynthetic protein-transferase complex, biosynthetic protein/transferase
• 由来する生物種: Pseudomonas aeruginosa; 詳細
• 細胞内の位置: Cytoplasm: P32324
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 348695.31

構造登録者

Joergensen, R.,Merrill, A.R.,Yates, S.P.,Marquez, V.E.,Schwan, A.L.,Boesen, T.,Andersen, G.R. (登録日: 2005-05-10, 公開日: 2005-05-24, 最終更新日: 2023-08-23)

主引用文献

Joergensen, R.,Merrill, A.R.,Yates, S.P.,Marquez, V.E.,Schwan, A.L.,Boesen, T.,Andersen, G.R.
Exotoxin A-eEF2 complex structure indicates ADP ribosylation by ribosome mimicry.
Nature, 436:979-984, 2005

PubMed Abstract: The bacteria causing diphtheria, whooping cough, cholera and other diseases secrete mono-ADP-ribosylating toxins that modify intracellular proteins. Here, we describe four structures of a catalytically active complex between a fragment of Pseudomonas aeruginosa exotoxin A (ETA) and its protein substrate, translation elongation factor 2 (eEF2). The target residue in eEF2, diphthamide (a modified histidine), spans across a cleft and faces the two phosphates and a ribose of the non-hydrolysable NAD+ analogue, betaTAD. This suggests that the diphthamide is involved in triggering NAD+ cleavage and interacting with the proposed oxacarbenium intermediate during the nucleophilic substitution reaction, explaining the requirement of diphthamide for ADP ribosylation. Diphtheria toxin may recognize eEF2 in a manner similar to ETA. Notably, the toxin-bound betaTAD phosphates mimic the phosphate backbone of two nucleotides in a conformational switch of 18S rRNA, thereby achieving universal recognition of eEF2 by ETA.

PubMed: 16107839
DOI: 10.1038/nature03871

実験手法

X-RAY DIFFRACTION (3.07 Å)