1ZIE
Hydrogenated gammaE crystallin in D2O solvent
Summary for 1ZIE
| Entry DOI | 10.2210/pdb1zie/pdb |
| Related | 1A5D 1ZGT 1ZIQ 1ZIR |
| Descriptor | Gamma crystallin E, ACETATE ION (3 entities in total) |
| Functional Keywords | 4 greek key motifs, structural protein |
| Biological source | Rattus norvegicus (Norway rat) |
| Total number of polymer chains | 1 |
| Total formula weight | 21281.70 |
| Authors | Artero, J.B.,Hartlein, M.,McSweeney, S.,Timmins, P. (deposition date: 2005-04-27, release date: 2005-11-08, Last modification date: 2023-10-25) |
| Primary citation | Artero, J.B.,Hartlein, M.,McSweeney, S.,Timmins, P. A comparison of refined X-ray structures of hydrogenated and perdeuterated rat gammaE-crystallin in H2O and D2O. Acta Crystallogr.,Sect.D, 61:1541-1549, 2005 Cited by PubMed Abstract: Rat gammaE-crystallin was overexpressed, purified under different labelling conditions and crystallized and X-ray data were collected at resolutions between 1.71 and 1.36 A. The structures were determined by molecular replacement. In these structures, the cd loop of the Greek-key motif 3, which is the major structural key motif of the two phase-transition groups of gamma-crystallins, presents a double conformation. The influence of the perdeuteration on the protein structure was determined by comparison of the atomic positions and temperature factors of the different models. The perdeuterated proteins have a similar structure to their hydrogenated counterparts, but partial or full deuteration may have some effect on the atomic B-factor values. PubMed: 16239733DOI: 10.1107/S0907444905028532 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.44 Å) |
Structure validation
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