1ZIA

OXIDIZED PSEUDOAZURIN

Summary for 1ZIA

• Entry DOI: 10.2210/pdb1zia/pdb
• Descriptor: PSEUDOAZURIN, COPPER (II) ION (3 entities in total)
• Functional Keywords: cuproprotein, electron transport
• Biological source: Achromobacter cycloclastes
• Cellular location: Periplasm: P19567
• Total number of polymer chains: 1
• Total formula weight: 13096.49

Authors

Inoue, T.,Nishio, N.,Hamanaka, S.,Shimomura, T.,Harada, S.,Suzuki, S.,Kohzuma, T.,Shidara, S.,Iwasaki, H.,Kai, Y. (deposition date: 1996-04-09, release date: 1997-04-01, Last modification date: 2024-02-14)

Primary citation

Inoue, T.,Nishio, N.,Kai, Y.,Harada, S.,Ohshiro, Y.,Suzuki, S.,Kohzuma, T.,Shidara, S.,Iwasaki, H.
Crystallization and preliminary X-ray studies on pseudoazurin from Achromobacter cycloclastes IAM1013.
J.Biochem.(Tokyo), 114:761-762, 1993

PubMed Abstract: New crystals of a blue copper protein, pseudoazurin from denitrifier Achromobacter cycloclastes IAM1013, have been obtained by means of vapor diffusion with ammonium sulfate as a precipitant at pH 6.0 and 4 degrees C. The crystals belong to the orthorhombic system, space group P2(1)2(1)2(1), with unit cell dimensions of a = 56.69(2), b = 61.53(2), and c = 30.20(1) A. The asymmetric unit includes one molecule of pseudoazurin with a Vm value of 2.04 A3/Da. The crystals are so stable against X-ray irradiation that a complete data set up to 1.54 A has been collected using a single native crystal. Solution of the structure was performed by means of the Patterson search techniques, and the current crystallographic R-factor is 17.5% at 3.0 A resolution. Refinement at higher resolution is in progress.

PubMed: 8138527

Experimental method

X-RAY DIFFRACTION (1.54 Å)