1ZHY

Structure of yeast oxysterol binding protein Osh4 in complex with cholesterol

Summary for 1ZHY

• Entry DOI: 10.2210/pdb1zhy/pdb
• Related: 1zht 1zhw 1zhx 1zhz 1zi7
• Descriptor: KES1 protein, LEAD (II) ION, CHOLESTEROL, ... (4 entities in total)
• Functional Keywords: oxysterol, sterol binding protein, lipid binding protein
• Biological source: Saccharomyces cerevisiae (baker's yeast)
• Total number of polymer chains: 1
• Total formula weight: 50699.28

Authors

Im, Y.J.,Raychaudhuri, S.,Prinz, W.A.,Hurley, J.H. (deposition date: 2005-04-26, release date: 2005-09-06, Last modification date: 2023-08-23)

Primary citation

Im, Y.J.,Raychaudhuri, S.,Prinz, W.A.,Hurley, J.H.
Structural mechanism for sterol sensing and transport by OSBP-related proteins
Nature, 437:154-158, 2005

PubMed Abstract: The oxysterol-binding-protein (OSBP)-related proteins (ORPs) are conserved from yeast to humans, and are implicated in the regulation of sterol homeostasis and in signal transduction pathways. Here we report the structure of the full-length yeast ORP Osh4 (also known as Kes1) at 1.5-1.9 A resolution in complexes with ergosterol, cholesterol, and 7-, 20- and 25-hydroxycholesterol. We find that a single sterol molecule binds within a hydrophobic tunnel in a manner consistent with a transport function for ORPs. The entrance is blocked by a flexible amino-terminal lid and surrounded by basic residues that are critical for Osh4 function. The structure of the open state of a lid-truncated form of Osh4 was determined at 2.5 A resolution. Structural analysis and limited proteolysis show that sterol binding closes the lid and stabilizes a conformation favouring transport across aqueous barriers and signal transmission. The structure of Osh4 in the absence of ligand exposes potential phospholipid-binding sites that are positioned for membrane docking and sterol exchange. On the basis of these observations, we propose a model in which sterol and membrane binding promote reciprocal conformational changes that facilitate a sterol transfer and signalling cycle.

PubMed: 16136145
DOI: 10.1038/nature03923

Experimental method

X-RAY DIFFRACTION (1.6 Å)