1ZES

BeF3- activated PhoB receiver domain

1ZES の概要

• エントリーDOI: 10.2210/pdb1zes/pdb
• 分子名称: Phosphate regulon transcriptional regulatory protein phoB, MAGNESIUM ION, BERYLLIUM TRIFLUORIDE ION, ... (4 entities in total)
• 機能のキーワード: chey-like fold, response regulator, transcription factor, phob, activated, transcription activator
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm: P08402
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 43304.42

構造登録者

Bachhawat, P.,Montelione, G.T.,Stock, A.M. (登録日: 2005-04-19, 公開日: 2005-09-20, 最終更新日: 2024-02-14)

主引用文献

Bachhawat, P.,Swapna, G.V.,Montelione, G.T.,Stock, A.M.
Mechanism of Activation for Transcription Factor PhoB Suggested by Different Modes of Dimerization in the Inactive and Active States.
Structure, 13:1353-1363, 2005

PubMed Abstract: Response regulators (RRs), which undergo phosphorylation/dephosphorylation at aspartate residues, are highly prevalent in bacterial signal transduction. RRs typically contain an N-terminal receiver domain that regulates the activities of a C-terminal DNA binding domain in a phosphorylation-dependent manner. We present crystallography and solution NMR data for the receiver domain of Escherichia coli PhoB which show distinct 2-fold symmetric dimers in the inactive and active states. These structures, together with the previously determined structure of the C-terminal domain of PhoB bound to DNA, define the conformation of the active transcription factor and provide a model for the mechanism of activation in the OmpR/PhoB subfamily, the largest group of RRs. In the active state, the receiver domains dimerize with 2-fold rotational symmetry using their alpha4-beta5-alpha5 faces, while the effector domains bind to DNA direct repeats with tandem symmetry, implying a loss of intramolecular interactions.

PubMed: 16154092
DOI: 10.1016/j.str.2005.06.006

実験手法

X-RAY DIFFRACTION (1.9 Å)