1ZE9

Zinc-binding domain of Alzheimer's disease amyloid beta-peptide complexed with a zinc (II) cation

1ZE9 の概要

• エントリーDOI: 10.2210/pdb1ze9/pdb
• 関連するPDBエントリー: 1ZE7 2BP4
• 分子名称: 16-mer from Alzheimer's disease amyloid Protein, ZINC ION (2 entities in total)
• 機能のキーワード: peptide-zinc complex, metal binding protein
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P05067
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2049.50

構造登録者

Zirah, S.,Kozin, S.A.,Mazur, A.K.,Blond, A.,Cheminant, M.,Segalas-Milazzo, I.,Debey, P.,Rebuffat, S. (登録日: 2005-04-18, 公開日: 2005-05-03, 最終更新日: 2024-11-20)

主引用文献

Zirah, S.,Kozin, S.A.,Mazur, A.K.,Blond, A.,Cheminant, M.,Segalas-Milazzo, I.,Debey, P.,Rebuffat, S.
Structural changes of region 1-16 of the Alzheimer disease amyloid beta-peptide upon zinc binding and in vitro aging.
J.Biol.Chem., 281:2151-2161, 2006

PubMed Abstract: Amyloid deposits within the cerebral tissue constitute a characteristic lesion associated with Alzheimer disease. They mainly consist of the amyloid peptide Abeta and display an abnormal content in Zn(2+) ions, together with many truncated, isomerized, and racemized forms of Abeta. The region 1-16 of Abeta can be considered the minimal zinc-binding domain and contains two aspartates subject to protein aging. The influence of zinc binding and protein aging related modifications on the conformation of this region of Abeta is of importance given the potentiality of this domain to constitute a therapeutic target, especially for immunization approaches. In this study, we determined from NMR data the solution structure of the Abeta-(1-16)-Zn(2+) complex in aqueous solution at pH 6.5. The residues His(6), His(13), and His(14) and the Glu(11) carboxylate were identified as ligands that tetrahedrally coordinate the Zn(II) cation. In vitro aging experiments on Abeta-(1-16) led to the formation of truncated and isomerized species. The major isomer generated, Abeta-(1-16)-l-iso-Asp(7), displayed a local conformational change in the His(6)-Ser(8) region but kept a zinc binding propensity via a coordination mode involving l-iso-Asp(7). These results are discussed here with regard to Abeta fibrillogenesis and the potentiality of the region 1-16 of Abeta to be used as a therapeutic target.

PubMed: 16301322
DOI: 10.1074/jbc.M504454200

実験手法

SOLUTION NMR