1ZBJ

Inferential Structure Determination of the Fyn SH3 domain using NOESY data from a 15N,H2 enriched protein

1ZBJ の概要

• エントリーDOI: 10.2210/pdb1zbj/pdb
• 関連するPDBエントリー: 1NYF 1SHF
• 分子名称: Proto-oncogene tyrosine-protein kinase FYN (1 entity in total)
• 機能のキーワード: transferase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cell membrane: P06241
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6738.28

構造登録者

Rieping, W.,Habeck, M.,Nilges, M. (登録日: 2005-04-08, 公開日: 2005-05-03, 最終更新日: 2024-05-22)

主引用文献

Rieping, W.,Habeck, M.,Nilges, M.
Inferential Structure Determination
Science, 309:303-306, 2005

PubMed Abstract: Macromolecular structures calculated from nuclear magnetic resonance data are not fully determined by experimental data but depend on subjective choices in data treatment and parameter settings. This makes it difficult to objectively judge the precision of the structures. We used Bayesian inference to derive a probability distribution that represents the unknown structure and its precision. This probability distribution also determines additional unknowns, such as theory parameters, that previously had to be chosen empirically. We implemented this approach by using Markov chain Monte Carlo techniques. Our method provides an objective figure of merit and improves structural quality.

PubMed: 16002620
DOI: 10.1126/science.1110428

実験手法

SOLUTION NMR