1ZBE

Foot-and Mouth Disease Virus Serotype A1061

1ZBE の概要

• エントリーDOI: 10.2210/pdb1zbe/pdb
• 関連するPDBエントリー: 1BBT 1FOD 1ZBA
• 分子名称: Coat protein VP1, Coat protein VP2, Coat protein VP3, ... (4 entities in total)
• 機能のキーワード: virus/viral protein, icosahedral virus, virus
• 由来する生物種: Foot-and-mouth disease virus; 詳細
• 細胞内の位置: Protein VP2: Virion. Protein VP3: Virion. Protein VP1: Virion. Protein 2B: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 2C: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 3A: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 3B-1: Virion (Potential). Protein 3B-2: Virion (Potential). Protein 3B-3: Virion (Potential). Picornain 3C: Host cytoplasm (Potential). RNA-directed RNA polymerase 3D-POL: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential): P03306 P03306 P03306 P03306
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 81000.39

構造登録者

Fry, E.E.,Newman, J.W.,Curry, S.,Najjam, S.,Jackson, T.,Blakemore, W.,Lea, S.M.,Miller, L.,Burman, A.,King, A.M.,Stuart, D.I. (登録日: 2005-04-08, 公開日: 2005-06-28, 最終更新日: 2024-02-14)

主引用文献

Fry, E.E.,Newman, J.W.,Curry, S.,Najjam, S.,Jackson, T.,Blakemore, W.,Lea, S.M.,Miller, L.,Burman, A.,King, A.M.,Stuart, D.I.
Structure of Foot-and-mouth disease virus serotype A1061 alone and complexed with oligosaccharide receptor: receptor conservation in the face of antigenic variation.
J.Gen.Virol., 86:1909-1920, 2005

PubMed Abstract: Foot-and-mouth disease viruses (FMDVs) target epithelial cells via integrin receptors, but can acquire the capacity to bind cell-surface heparan sulphate (or alternative receptors) on passage in cell culture. Vaccine viruses must be propagated in cell culture and, hence, some rationale for the selection of variants in this process is important. Crystal structures are available for type O, A and C viruses and also for a complex of type O strain O(1)BFS with heparin. The structure of FMDV A10(61) (a cell culture-adapted strain) complexed with heparin has now been determined. This virus has an RGSD motif in place of the otherwise conserved RGD integrin-binding motif and the potential to bind heparan sulphate (suggested by sequence analyses). FMDV A10(61) was closely similar in structure to other serotypes, deviating most in antigenic sites. The VP1 GH loop comprising the integrin-binding motif was disordered. Heparin bound at a similar site and in a similar conformation to that seen in the analogous complex with O(1)BFS, although the binding had a lower affinity and was more ionic.

PubMed: 15958669
DOI: 10.1099/vir.0.80730-0

実験手法

X-RAY DIFFRACTION (3 Å)