1ZBA

Foot-and-Mouth Disease virus serotype A1061 complexed with oligosaccharide receptor.

Summary for 1ZBA

• Entry DOI: 10.2210/pdb1zba/pdb
• Related: 1qqp 1zbe
• Descriptor: Coat protein VP1, Coat protein VP2, Coat protein VP3, ... (6 entities in total)
• Functional Keywords: oligosaccharide receptor, virus/viral protein, icosahedral virus, virus
• Biological source: Foot-and-mouth disease virus; More
• Cellular location: Protein VP2: Virion. Protein VP3: Virion. Protein VP1: Virion. Protein 2B: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 2C: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 3A: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 3B-1: Virion (Potential). Protein 3B-2: Virion (Potential). Protein 3B-3: Virion (Potential). Picornain 3C: Host cytoplasm (Potential). RNA-directed RNA polymerase 3D-POL: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential): Q84769 Q84769 Q84769 Q84769
• Total number of polymer chains: 4
• Total formula weight: 81917.16

Authors

Fry, E.E.,Newman, J.W.,Curry, S.,Najjam, S.,Jackson, T.,Blakemore, W.,Lea, S.M.,Miller, L.,Burman, A.,King, A.M.,Stuart, D.I. (deposition date: 2005-04-08, release date: 2005-06-28, Last modification date: 2024-02-14)

Primary citation

Fry, E.E.,Newman, J.W.,Curry, S.,Najjam, S.,Jackson, T.,Blakemore, W.,Lea, S.M.,Miller, L.,Burman, A.,King, A.M.,Stuart, D.I.
Structure of Foot-and-mouth disease virus serotype A1061 alone and complexed with oligosaccharide receptor: receptor conservation in the face of antigenic variation.
J.Gen.Virol., 86:1909-1920, 2005

PubMed Abstract: Foot-and-mouth disease viruses (FMDVs) target epithelial cells via integrin receptors, but can acquire the capacity to bind cell-surface heparan sulphate (or alternative receptors) on passage in cell culture. Vaccine viruses must be propagated in cell culture and, hence, some rationale for the selection of variants in this process is important. Crystal structures are available for type O, A and C viruses and also for a complex of type O strain O(1)BFS with heparin. The structure of FMDV A10(61) (a cell culture-adapted strain) complexed with heparin has now been determined. This virus has an RGSD motif in place of the otherwise conserved RGD integrin-binding motif and the potential to bind heparan sulphate (suggested by sequence analyses). FMDV A10(61) was closely similar in structure to other serotypes, deviating most in antigenic sites. The VP1 GH loop comprising the integrin-binding motif was disordered. Heparin bound at a similar site and in a similar conformation to that seen in the analogous complex with O(1)BFS, although the binding had a lower affinity and was more ionic.

PubMed: 15958669
DOI: 10.1099/vir.0.80730-0

Experimental method

X-RAY DIFFRACTION (2 Å)