1ZAE

Solution structure of the functional domain of phi29 replication organizer p16.7c

「1Z61」から置き換えられました

1ZAE の概要

• エントリーDOI: 10.2210/pdb1zae/pdb
• 関連するPDBエントリー: 2BNK
• 分子名称: Early protein GP16.7 (1 entity in total)
• 機能のキーワード: phi-29 replication, nonspecific dna binding protein, helical dimer, dna binding protein
• 由来する生物種: Bacillus phage phi29
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 16644.80

構造登録者

Asensio, J.L.,Albert, A.,Munoz-Espin, D.,Gonzalez, C.,Hermoso, J.,Villar, L.,Jimenez-Barbero, J.,Salas, M.,Meijer, W.J.J. (登録日: 2005-04-06, 公開日: 2005-04-19, 最終更新日: 2024-05-29)

主引用文献

Asensio, J.L.,Albert, A.,Munoz-Espin, D.,Gonzalez, C.,Hermoso, J.,Villar, L.,Jimenez-Barbero, J.,Salas, M.,Meijer, W.J.J.
Structure of the functional domain of phi29 replication organizer: insights into oligomerization and dna binding
J.Biol.Chem., 280:20730-20739, 2005

PubMed Abstract: The Bacillus subtilis phage phi29-encoded membrane protein p16.7 is one of the few proteins involved in prokaryotic membrane-associated DNA replication that has been characterized at a functional and biochemical level. In this work we have determined both the solution and crystal structures of its dimeric functional domain, p16.7C. Although the secondary structure of p16.7C is remarkably similar to that of the DNA binding homeodomain, present in proteins belonging to a large family of eukaryotic transcription factors, the tertiary structures of p16.7C and homeodomains are fundamentally different. In fact, p16.7C defines a novel dimeric six-helical fold. We also show that p16.7C can form multimers in solution and that this feature is a key factor for efficient DNA binding. Moreover, a combination of NMR and x-ray approaches, combined with functional analyses of mutants, revealed that multimerization of p16.7C dimers is mediated by a large protein surface that is characterized by a striking self-complementarity. Finally, the structural analyses of the p16.7C dimer and oligomers provide important clues about how protein multimerization and DNA binding are coupled.

PubMed: 15772069
DOI: 10.1074/jbc.M501687200

実験手法

SOLUTION NMR