1ZAA

ZINC FINGER-DNA RECOGNITION: CRYSTAL STRUCTURE OF A ZIF268-DNA COMPLEX AT 2.1 ANGSTROMS

1ZAA の概要

• エントリーDOI: 10.2210/pdb1zaa/pdb
• 分子名称: DNA (5'-D(*AP*GP*CP*GP*TP*GP*GP*GP*CP*GP*T)-3'), DNA (5'-D(*TP*AP*CP*GP*CP*CP*CP*AP*CP*GP*C)-3'), PROTEIN (ZIF268), ... (5 entities in total)
• 機能のキーワード: protein-dna complex, double helix, transcription-dna complex, transcription/dna
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Nucleus: P08046
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 17393.66

構造登録者

Pavletich, N.P.,Pabo, C.O. (登録日: 1992-09-17, 公開日: 1993-10-31, 最終更新日: 2024-02-14)

主引用文献

Pavletich, N.P.,Pabo, C.O.
Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A.
Science, 252:809-817, 1991

PubMed Abstract: The zinc finger DNA-binding motif occurs in many proteins that regulate eukaryotic gene expression. The crystal structure of a complex containing the three zinc fingers from Zif268 (a mouse immediate early protein) and a consensus DNA-binding site has been determined at 2.1 angstroms resolution and refined to a crystallographic R factor of 18.2 percent. In this complex, the zinc fingers bind in the major groove of B-DNA and wrap part way around the double helix. Each finger has a similar relation to the DNA and makes its primary contacts in a three-base pair subsite. Residues from the amino-terminal portion of an alpha helix contact the bases, and most of the contracts are made with the guanine-rich strand of the DNA. This structure provides a framework for understanding how zinc fingers recognize DNA and suggests that this motif may provide a useful basis for the design of novel DNA-binding proteins.

PubMed: 2028256

実験手法

X-RAY DIFFRACTION (2.1 Å)