1Z9B

Solution structure of the C1-subdomain of Bacillus stearothermophilus translation initiation factor IF2

1Z9B の概要

• エントリーDOI: 10.2210/pdb1z9b/pdb
• 関連するPDBエントリー: 1D1N
• 分子名称: Translation initiation factor IF-2 (1 entity in total)
• 機能のキーワード: protein synthesis translation intiation if2 fmet-trna nmr structure, translation
• 由来する生物種: Geobacillus stearothermophilus
• 細胞内の位置: Cytoplasm: P04766
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14815.85

構造登録者

Wienk, H.,Tomaselli, S.,Bernard, C.,Spurio, R.,Picone, D.,Gualerzi, C.O.,Boelens, R. (登録日: 2005-04-01, 公開日: 2005-08-30, 最終更新日: 2024-05-22)

主引用文献

Wienk, H.,Tomaselli, S.,Bernard, C.,Spurio, R.,Picone, D.,Gualerzi, C.O.,Boelens, R.
Solution structure of the C1-subdomain of Bacillus stearothermophilus translation initiation factor IF2
Protein Sci., 14:2461-2468, 2005

PubMed Abstract: IF2 is one of three bacterial translation initiation factors that are conserved through all kingdoms of life. It binds the 30S and 50S ribosomal subunits, as well as fMet-tRNAf(Met). After these interactions, fMet-tRNAf(Met) is oriented to the ribosomal P-site where the first amino acid of the nascent polypeptide, formylmethionine, is presented. The C-terminal domain of Bacillus stearothermophilus IF2, which is responsible for recognition and binding of fMet-tRNAf(Met), contains two structured modules. Previously, the solution structure of the most C-terminal module, IF2-C2, has been elucidated by NMR spectroscopy and direct interactions between this subdomain and fMet-tRNAf(Met) were reported. In the present NMR study we have obtained the spectral assignment of the other module of the C-terminal domain (IF2-C1) and determined its solution structure and backbone dynamics. The IF2-C1 core forms a flattened fold consisting of a central four-stranded parallel beta-sheet flanked by three alpha-helices. Although its overall organization resembles that of subdomain III of the archaeal IF2-homolog eIF5B whose crystal structure had previously been reported, some differences of potential functional significance are evident.

PubMed: 16081655
DOI: 10.1110/ps.051531305

実験手法

SOLUTION NMR