1Z70

1.15A resolution structure of the formylglycine generating enzyme FGE

1Z70 の概要

• エントリーDOI: 10.2210/pdb1z70/pdb
• 関連するPDBエントリー: 1y1e 1y1f 1y1g 1y1h 1y1i 1y1j
• 分子名称: C-alpha-formyglycine-generating enzyme, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, CALCIUM ION, ... (6 entities in total)
• 機能のキーワード: formylglycine, multiple sulfatase deficiency, cysteine sulfenic acid, oxidoreductase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35765.97

構造登録者

Rudolph, M.G. (登録日: 2005-03-23, 公開日: 2005-07-22, 最終更新日: 2023-11-15)

主引用文献

Roeser, D.,Dickmanns, A.,Gasow, K.,Rudolph, M.G.
De novo calcium/sulfur SAD phasing of the human formylglycine-generating enzyme using in-house data.
Acta Crystallogr.,Sect.D, 61:1057-1066, 2005

PubMed Abstract: Sulfatases are a family of enzymes essential for the degradation of sulfate esters. Formylglycine is the key catalytic residue in the active site of sulfatases and is generated from a cysteine residue by FGE, the formylglycine-generating enzyme. Inactivity of FGE owing to inherited mutations in the FGE gene results in multiple sulfatase deficiency (MSD), which leads to early death in infants. Human FGE was crystallized in the presence of traces of the protease elastase, which was absolutely essential for crystal growth, and the structure of FGE was determined by molecular replacement. Before this model was completed, the FGE structure was re-determined by SAD phasing using in-house data based on the anomalous signal of two calcium ions bound to the native enzyme and intrinsic S atoms. A 14-atom substructure was determined at 1.8 A resolution by SHELXD; SHELXE was used for density modification and phase extension to 1.54 A resolution. Automated model building with ARP/wARP and refinement with REFMAC5 yielded a virtually complete model without manual intervention. The minimal data requirements for successful phasing and the relative contributions of the Ca and S atoms are discussed and compared with the related FGE paralogue, pFGE. This work emphasizes the usefulness of de novo phasing using weak anomalous scatterers and in-house data.

PubMed: 16041070
DOI: 10.1107/S0907444905013831

実験手法

X-RAY DIFFRACTION (1.15 Å)