1Z6D

Ribonuclease A- IMP complex

1Z6D の概要

• エントリーDOI: 10.2210/pdb1z6d/pdb
• 関連するPDBエントリー: 1O0F 1O0H 1O0M 1O0N 1O0O 1Z6S
• 分子名称: Ribonuclease pancreatic, INOSINIC ACID (3 entities in total)
• 機能のキーワード: hydrolase, ribonuclease
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Secreted: P61823
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29157.68

構造登録者

Hatzopoulos, G.N.,Leonidas, D.D.,Kardakaris, R.,Kobe, J.,Oikonomakos, N.G. (登録日: 2005-03-22, 公開日: 2005-08-16, 最終更新日: 2024-10-30)

主引用文献

Hatzopoulos, G.N.,Leonidas, D.D.,Kardakaris, R.,Kobe, J.,Oikonomakos, N.G.
The binding of IMP to Ribonuclease A
Febs J., 272:3988-4001, 2005

PubMed Abstract: The binding of inosine 5' phosphate (IMP) to ribonuclease A has been studied by kinetic and X-ray crystallographic experiments at high (1.5 A) resolution. IMP is a competitive inhibitor of the enzyme with respect to C>p and binds to the catalytic cleft by anchoring three IMP molecules in a novel binding mode. The three IMP molecules are connected to each other by hydrogen bond and van der Waals interactions and collectively occupy the B1R1P1B2P0P(-1) region of the ribonucleolytic active site. One of the IMP molecules binds with its nucleobase in the outskirts of the B2 subsite and interacts with Glu111 while its phosphoryl group binds in P1. Another IMP molecule binds by following the retro-binding mode previously observed only for guanosines with its nucleobase at B1 and the phosphoryl group in P(-1). The third IMP molecule binds in a novel mode towards the C-terminus. The RNase A-IMP complex provides structural evidence for the functional components of subsite P(-1) while it further supports the role inferred by other studies to Asn71 as the primary structural determinant for the adenine specificity of the B2 subsite. Comparative structural analysis of the IMP and AMP complexes highlights key aspects of the specificity of the base binding subsites of RNase A and provides a structural explanation for their potencies. The binding of IMP suggests ways to develop more potent inhibitors of the pancreatic RNase superfamily using this nucleotide as the starting point.

PubMed: 16045769
DOI: 10.1111/j.1742-4658.2005.04822.x

実験手法

X-RAY DIFFRACTION (1.54 Å)