1Z56

Co-Crystal Structure of Lif1p-Lig4p

Summary for 1Z56

• Entry DOI: 10.2210/pdb1z56/pdb
• Descriptor: Ligase interacting factor 1, DNA ligase IV, ... (9 entities in total)
• Functional Keywords: dna repair, brct, nhej, xrcc4, dna ligase, coiled-coil, ligase
• Biological source: Saccharomyces cerevisiae (baker's yeast); More
• Cellular location: Cytoplasm : P53150; Nucleus : Q08387
• Total number of polymer chains: 11
• Total formula weight: 102072.38

Authors

Dore, A.S.,Furnham, N.,Davies, O.R.,Sibanda, B.L.,Chirgadze, D.Y.,Jackson, S.P.,Pellegrini, L.,Blundell, T.L. (deposition date: 2005-03-17, release date: 2006-01-31, Last modification date: 2024-02-14)

Primary citation

Dore, A.S.,Furnham, N.,Davies, O.R.,Sibanda, B.L.,Chirgadze, D.Y.,Jackson, S.P.,Pellegrini, L.,Blundell, T.L.
Structure of an Xrcc4-DNA ligase IV yeast ortholog complex reveals a novel BRCT interaction mode.
DNA REPAIR, 5:362-368, 2006

PubMed Abstract: DNA ligase IV catalyses the final ligation step in the non-homologous end-joining (NHEJ) DNA repair pathway and requires interaction of the ligase with the Xrcc4 'genome-guardian', an essential NHEJ factor. Here we report the 3.9 A crystal structure of the Saccharomyces cerevisiae Xrcc4 ortholog ligase interacting factor 1 (Lif1p) complexed with the C-terminal BRCT domains of DNA ligase IV (Lig4p). The structure reveals a novel mode of protein recognition by a tandem BRCT repeat, and in addition provides a molecular basis for a human LIG4 syndrome clinical condition.

PubMed: 16388993
DOI: 10.1016/j.dnarep.2005.11.004

Experimental method

X-RAY DIFFRACTION (3.92 Å)