1Z53

The 1.13 Angstrom Structure of Iron-free Cytochrome c Peroxidase

1Z53 の概要

• エントリーDOI: 10.2210/pdb1z53/pdb
• 関連するPDBエントリー: 1KOK 2CYP
• 分子名称: Cytochrome c peroxidase, mitochondrial, PROTOPORPHYRIN IX (3 entities in total)
• 機能のキーワード: ccp, heme peroxidase, iron-free protoporphyrin ix, trp cation radical, oxidoreductase
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Mitochondrion matrix: P00431
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34133.90

構造登録者

Bhaskar, B.,Poulos, T.L. (登録日: 2005-03-17, 公開日: 2005-06-21, 最終更新日: 2024-04-03)

主引用文献

Bhaskar, B.,Poulos, T.L.
The 1.13-A structure of iron-free cytochrome c peroxidase.
J.Biol.Inorg.Chem., 10:425-430, 2005

PubMed Abstract: The iron-free cytochrome c peroxidase (CCP) crystal structure has been determined to 1.13 A and compared with the 1.2-A ferric-CCP structure. Quite unexpectedly, removal of the iron has no effect on porphyrin geometry and distortion, indicating that protein-porphyrin interactions and not iron coordination or formation of the axial His-Fe bond determines porphyrin conformation. However, there are changes in solvent structure in the distal pocket, which lead to changes in the distal His52 acid-base catalyst. The observed ability of His52 to move in response to small changes in solvent structure is very likely important for its role as a catalyst in assisting in the heterolytic fission of the peroxide O-O bond.

PubMed: 15900441
DOI: 10.1007/s00775-005-0654-4

実験手法

X-RAY DIFFRACTION (1.13 Å)