1Z4Z

Parainfluenza Virus 5 (SV5) Hemagglutinin-Neuraminidase (HN) with ligand DANA(soaked with sialic acid, pH7.0))

1Z4Z の概要

• エントリーDOI: 10.2210/pdb1z4z/pdb
• 関連するPDBエントリー: 1Z4V 1Z4W 1Z4X 1Z4Y 1Z50
• 分子名称: Hemagglutinin-neuraminidase, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: hemagglutinin, neuraminidase, fusion, tetramer, hydrolase
• 由来する生物種: Simian virus 5
• 細胞内の位置: Virion membrane; Single-pass type II membrane protein (Potential): P04850
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 59910.15

構造登録者

Yuan, P.,Thompson, T.B.,Wurzburg, B.A.,Paterson, R.G.,Lamb, R.A.,Jardetzky, T.S. (登録日: 2005-03-16, 公開日: 2005-05-24, 最終更新日: 2024-10-30)

主引用文献

Yuan, P.,Thompson, T.B.,Wurzburg, B.A.,Paterson, R.G.,Lamb, R.A.,Jardetzky, T.S.
Structural studies of the parainfluenza virus 5 hemagglutinin-neuraminidase tetramer in complex with its receptor, sialyllactose.
Structure, 13:803-815, 2005

PubMed Abstract: The paramyxovirus hemagglutinin-neuraminidase (HN) functions in virus attachment to cells, cleavage of sialic acid from oligosaccharides, and stimulating membrane fusion during virus entry into cells. The structural basis for these diverse functions remains to be fully understood. We report the crystal structures of the parainfluenza virus 5 (SV5) HN and its complexes with sialic acid, the inhibitor DANA, and the receptor sialyllactose. SV5 HN shares common structural features with HN of Newcastle disease virus (NDV) and human parainfluenza 3 (HPIV3), but unlike the previously determined HN structures, the SV5 HN forms a tetramer in solution, which is thought to be the physiological oligomer. The sialyllactose complex reveals intact receptor within the active site, but no major conformational changes in the protein. The SV5 HN structures do not support previously proposed models for HN action in membrane fusion and suggest alternative mechanisms by which HN may promote virus entry into cells.

PubMed: 15893670
DOI: 10.1016/j.str.2005.02.019

実験手法

X-RAY DIFFRACTION (2.5 Å)